CAR Antibody (Mab.E[mh] 1): sc-32795

CAR Antibody (Mab.E[mh] 1) is a mouse monoclonal IgG1 kappa light chain antibody that detects the coxsackie and adenovirus receptor (CAR) protein of mouse, rat, and human origin by western blotting (WB) and immunoprecipitation (IP). Anti-CAR antibody (Mab.E[mh] 1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The coxsackie and adenovirus receptor plays a crucial role in mediating viral infections by facilitating the binding of various adenoviruses through specific protein interactions. Notably, the high affinity between the viral knob domain and the extracellular amino-terminal domain (designated D1) of CAR is significant, as the D1 domain alone is sufficient for knob binding in transfected cells. Understanding the structural dynamics of CAR is essential, as CAR not only influences the efficiency of adenoviral infection but also has implications for the development of targeted gene therapies. CAR is expressed in a variety of human and murine cell types, but cells with low CAR expression levels are less susceptible to adenoviral infection. Strategies to enhance CAR expression or modify the adenoviral knob to target alternative receptors are being explored to overcome these limitations, making CAR monoclonal antibody (Mab.E[mh] 1) a valuable tool for researchers investigating viral interactions and therapeutic applications.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CAR Antibody (Mab.E[mh] 1) References:

1. Downregulation of the coxsackie and adenovirus receptor in cancer cells by hypoxia depends on HIF-1alpha.  |  Küster, K., et al. 2010. Cancer Gene Ther. 17: 141-6. PMID: 19590529
2. Research advance on role of Coxsackie and adenovirus receptor (CAR) in tumor progression.  |  Fan, LS., et al. 2009. Ai Zheng. 28: 333-6. PMID: 19619453
3. Essential role of the coxsackie- and adenovirus receptor (CAR) in development of the lymphatic system in mice.  |  Mirza, M., et al. 2012. PLoS One. 7: e37523. PMID: 22624044
4. Coxsackie and adenovirus receptor is a modifier of cardiac conduction and arrhythmia vulnerability in the setting of myocardial ischemia.  |  Marsman, RF., et al. 2014. J Am Coll Cardiol. 63: 549-59. PMID: 24291282
5. Regulation of the Coxsackie and adenovirus receptor expression is dependent on cystic fibrosis transmembrane regulator in airway epithelial cells.  |  Sharma, A., et al. 2017. Cell Microbiol. 19: PMID: 27527752
6. Adenovirus coxsackie adenovirus receptor-mediated binding to human erythrocytes does not preclude systemic transduction.  |  Rojas, LA., et al. 2016. Cancer Gene Ther. 23: 411-414. PMID: 27767087
7. ERK1/2 pathway regulates coxsackie and adenovirus receptor expression in mouse cardiac stem cells.  |  Liu, J., et al. 2017. Exp Ther Med. 13: 3348-3354. PMID: 28587412
8. Expression of coxsackie and adenovirus receptor is correlated with inferior prognosis in liver cancer patients.  |  Yang, X., et al. 2019. Oncol Lett. 17: 2485-2490. PMID: 30719117
9. Human Coxsackie- and adenovirus receptor is a putative target of neutrophil elastase-mediated shedding.  |  Herrmann, L., et al. 2022. Mol Biol Rep. 49: 3213-3223. PMID: 35122600
10. Experimental infection of foot and mouth disease virus (FMDV) upregulates the expression of Coxsackie and adenovirus receptor (CAR) in the myocardium of suckling mice.  |  Priyanka, M., et al. 2023. Microb Pathog. 184: 106383. PMID: 37806501

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CAR Antibody (Mab.E[mh] 1) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-CAR Antibody (Mab.E[mh] 1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CAR Antibody (Mab.E[mh] 1) Specifications

Alternate Product Name: CAR

Product Clone Name: Mab.E[mh] 1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: CAR

Epitope Species: human

Product Reactivity/Detection: CAR

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for CAR Antibody (Mab.E[mh] 1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CAR antibody is CAR Antibody (E-1): sc-373791.