Cappuccino CRISPR/Cas9 KO Plasmid (h): sc-407008
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cappuccino CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Cappuccino genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cappuccino CRISPR/Cas9 KO Plasmid (h) | sc-407008 | 20 µg | $397.00 |
Overview
Human BLOC1S4 encodes cappuccino, a core component of the biogenesis of lysosome-related organelles complex 1 (BLOC-1), which coordinates endosomal membrane trafficking and cargo sorting required for specialized organelle maturation. BLOC-1 function supports vesicle budding, recycling, and delivery of protein cargo through the endosome–lysosome system, impacting processes such as pigment granule biogenesis and secretory pathways in specialized cells. Disruption of BLOC-1 subunits perturbs intracellular transport and vesicular dynamics, with relevance to disorders involving lysosome-related organelles and broader neurodevelopmental and synaptic phenotypes linked to endosomal trafficking dysfunction. As a result, BLOC1S4 is commonly studied in the context of endosomal sorting, organelle biogenesis, and pathway-level consequences of altered membrane trafficking.
Cappuccino CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the BLOC1S4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the BLOC1S4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the BLOC1S4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Cappuccino protein expression.
This CRISPR knockout system enables efficient generation of BLOC1S4-deficient cell models for investigation of Cappuccino signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting BLOC1S4 exon(s) critical for Cappuccino function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple BLOC1S4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Cappuccino CRISPR/Cas9 KO Plasmid (h) and Cappuccino CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the BLOC1S4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Cappuccino HDR Plasmid (h) and Cappuccino HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by BLOC1S4 homology arms to support homology-directed repair at defined BLOC1S4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.