CAP Antibody (G-3): sc-166903

CAP (G-3) is a mouse monoclonal IgG3 kappa light chain antibody that detects CAP protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-CAP antibody (G-3) is available as a non-conjugated form. c-Cbl associated protein (CAP), also designated ponsin and SH3P12, interacts with c-Cbl and facilitates c-Cbl tyrosine phosphorylation in response to insulin. CAP contains three adjacent Src homology-3 (SH3) domains in the carboxy terminus and interacts with focal adhesion kinase p125FAK, co-localizing with Actin stress fibers. CAP is expressed in 3T3-L1 adipocytes, but not in 3T3-L1 or NIH/3T3 fibroblasts. Thiazolidinediones (TZDs) stimulate CAP gene expression through PPARg activation. Beyond c-Cbl interaction, CAP interacts with Sos through the same SH3 domain, potentially facilitating protein-protein associations involved in cell structural changes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CAP Antibody (G-3) References:

1. Cloning and characterization of a functional peroxisome proliferator activator receptor-gamma-responsive element in the promoter of the CAP gene.  |  Baumann, CA., et al. 2000. J Biol Chem. 275: 9131-5. PMID: 10734046
2. A novel, multifuntional c-Cbl binding protein in insulin receptor signaling in 3T3-L1 adipocytes.  |  Ribon, V., et al. 1998. Mol Cell Biol. 18: 872-9. PMID: 9447983
3. A role for CAP, a novel, multifunctional Src homology 3 domain-containing protein in formation of actin stress fibers and focal adhesions.  |  Ribon, V., et al. 1998. J Biol Chem. 273: 4073-80. PMID: 9461600
4. Thiazolidinediones and insulin resistance: peroxisome proliferatoractivated receptor gamma activation stimulates expression of the CAP gene.  |  Ribon, V., et al. 1998. Proc Natl Acad Sci U S A. 95: 14751-6. PMID: 9843961
5. Molecular recognition properties of the C-terminal Sh3 domain of the Cbl associated protein, Cap.  |  Kurakin, A., et al. 1998. J Pept Res. 52: 331-7. PMID: 9894838

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CAP Antibody (G-3) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-CAP Antibody (G-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CAP Antibody (G-3) Specifications

Alternate Product Name: CAP

Product Clone Name: G-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 1184-1200

Epitope Target: CAP

Epitope Species: mouse

Product Reactivity/Detection: CAP

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for CAP Antibody (G-3): P: Blocking Peptide