CAML Double Nickase Plasmid (h): sc-403690-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CAML Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CAML Double Nickase Plasmid (h) and CAML Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CAMLG. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CAML Antibody (B-12): sc-166557
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CAML Double Nickase Plasmid (h) | sc-403690-NIC | 20 µg | $410.00 | |||
CAML Double Nickase Plasmid (h2) | sc-403690-NIC-2 | 20 µg | $410.00 |
CAMLG encodes calcium-modulating cyclophilin ligand (CAML), an endoplasmic reticulum membrane-associated protein that functions as a regulator of intracellular Ca²⁺ signaling and membrane protein biogenesis. CAML participates in processes linked to receptor-evoked calcium influx, ER homeostasis, and trafficking or stabilization of select membrane proteins, thereby influencing downstream pathways governing cell activation, survival, and stress responses. It has been studied in the context of immune cell signaling and general ER-associated regulatory networks that shape proteostasis. Dysregulated calcium handling and ER stress pathways that intersect with CAML-associated mechanisms are relevant to diverse disease biology, including immune dysfunction and cancer-associated signaling adaptations.
CAML Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CAMLG locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CAMLG. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CAMLG function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CAMLG-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.