Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CaMKIV Double Nickase Plasmid (h): sc-400806-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CaMKIV Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CaMKIV Double Nickase Plasmid (h) and CaMKIV Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CAMK4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CaMKIV Antibody (H-5): sc-55501
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CaMKIV Double Nickase Plasmid (h) | sc-400806-NIC | 20 µg | $410.00 | |||
CaMKIV Double Nickase Plasmid (h2) | sc-400806-NIC-2 | 20 µg | $410.00 |
CAMK4 encodes calcium/calmodulin-dependent protein kinase IV (CaMKIV), a serine/threonine kinase that transduces Ca2+ signals from calmodulin to nuclear transcriptional programs. CaMKIV is activated downstream of CaMKK and regulates phosphorylation of transcription factors such as CREB and MEF2, linking calcium dynamics to gene expression, neuronal plasticity, and activity-dependent differentiation. The kinase participates in pathways controlling immune cell activation and cytokine-related transcriptional responses, and its dysregulation has been studied in contexts including neurodevelopmental and neuropsychiatric phenotypes as well as immune-associated disorders. As a nuclear Ca2+-responsive regulator, CaMKIV is frequently used to interrogate stimulus-coupled transcription and chromatin-associated signaling in human cell models.
CaMKIV Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CAMK4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CAMK4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CAMK4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CAMK4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.