CAMK2A/CaMKII alpha Double Nickase Plasmid (h): sc-400228-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CAMK2A/CaMKII alpha Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CAMK2A/CaMKII alpha Double Nickase Plasmid (h) and CAMK2A/CaMKII alpha Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CAMK2A. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CAMK2A/CaMKII alpha Antibody (A-1): sc-13141
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CAMK2A/CaMKII alpha Double Nickase Plasmid (h) | sc-400228-NIC | 20 µg | $410.00 | |||
CAMK2A/CaMKII alpha Double Nickase Plasmid (h2) | sc-400228-NIC-2 | 20 µg | $410.00 |
CAMK2A encodes the alpha subunit of Ca2+/calmodulin-dependent protein kinase II (CaMKIIα), a serine/threonine kinase that decodes intracellular Ca2+ transients into phosphorylation events critical for synaptic transmission and activity-dependent plasticity. CaMKIIα undergoes autophosphorylation to sustain kinase activity and modulates glutamatergic signaling through phosphorylation of ion channels, receptors, and scaffolding proteins, integrating Ca2+ signaling with cytoskeletal remodeling and gene-expression programs. This kinase is a central node in neuronal signaling pathways that influence learning and memory, and altered CAMK2A activity has been associated with neurodevelopmental and neuropsychiatric phenotypes as well as seizure-related mechanisms in experimental models. Consequently, CAMK2A is widely used as a molecular handle to study excitatory synapse regulation, calcium-dependent signaling dynamics, and downstream phosphorylation networks in human neuronal systems.
CAMK2A/CaMKII alpha Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CAMK2A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CAMK2A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CAMK2A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CAMK2A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.