CaM III CRISPR/Cas9 KO Plasmid (h): sc-400953
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CaM III CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CaM III genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CaM III CRISPR/Cas9 KO Plasmid (h) | sc-400953 | 20 µg | $397.00 |
Overview
CALM3 encodes calmodulin III (CaM III), a highly conserved Ca2+ sensor that binds and regulates numerous effector proteins, including CaM-dependent kinases and phosphatases, ion channels, and components of the cytoskeleton. By translating intracellular calcium transients into changes in enzymatic activity, CaM III supports core processes such as excitation–contraction coupling, vesicle trafficking, transcriptional regulation, and cell cycle progression. CALM3-dependent signaling interfaces with pathways including CaMK and calcineurin/NFAT, shaping stimulus-responsive gene expression and cellular stress adaptation. Dysregulated calcium/calmodulin signaling has been linked to pathological remodeling in cardiovascular and neurological contexts, making CALM3 perturbation useful for mechanistic studies of Ca2+-driven phenotypes.
CaM III CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CALM3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CALM3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CALM3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CaM III protein expression.
This CRISPR knockout system enables efficient generation of CALM3-deficient cell models for investigation of CaM III signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CALM3 exon(s) critical for CaM III function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CALM3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CaM III CRISPR/Cas9 KO Plasmid (h) and CaM III CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CALM3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CaM III HDR Plasmid (h) and CaM III HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CALM3 homology arms to support homology-directed repair at defined CALM3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.