Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Calregulin CRISPR/Cas9 KO Plasmid (h): sc-400775
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Calregulin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Calregulin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Calregulin Antibody (F-4): sc-373863
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Calregulin CRISPR/Cas9 KO Plasmid (h) | sc-400775 | 20 µg | $397.00 |
Overview
CALR encodes calregulin, a multifunctional endoplasmic reticulum (ER) chaperone and high-capacity Ca²⁺-binding protein that supports protein folding quality control and intracellular calcium homeostasis. Calregulin participates in the calnexin/calregulin cycle, coordinating glycoprotein maturation and ER retention, and contributes to ER stress signaling and the unfolded protein response. Beyond the ER, CALR influences antigen presentation through MHC class I assembly, modulates cell adhesion and migration via integrin-related processes, and interfaces with Ca²⁺-dependent signaling pathways. Dysregulated CALR activity has been linked to altered proteostasis and immune signaling in diverse disease contexts, including hematologic and solid tumor biology, making it a useful node for mechanistic studies of ER function and cell-state regulation.
Calregulin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CALR gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CALR together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CALR open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Calregulin protein expression.
This CRISPR knockout system enables efficient generation of CALR-deficient cell models for investigation of Calregulin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CALR exon(s) critical for Calregulin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CALR genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Calregulin CRISPR/Cas9 KO Plasmid (h) and Calregulin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CALR locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Calregulin HDR Plasmid (h) and Calregulin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CALR homology arms to support homology-directed repair at defined CALR target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.