Calponin 1/2/3 Antibody (H-3): sc-133188

Calponin 1/2/3 Antibody (H-3) is a mouse monoclonal IgG3 antibody that detects Calponin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Calponin is a crucial actin- and tropomyosin-binding protein that plays a significant role in regulating smooth muscle contraction and serves as a marker for smooth muscle cell differentiation. Calponin primary function is to inhibit smooth muscle actomyosin activity, which is vital for maintaining proper muscle tone and function. Calponin achieves this by interacting with F-Actin and inhibiting the actin-activated MgATPase activity of phosphorylated myosin, thereby modulating contraction dynamics. Notably, Calponin regulatory properties are compromised upon phosphorylation, particularly at Serine 175, by protein kinase C or calmodulin-dependent protein kinase II, which can lead to altered muscle contractility. The three isoforms—Calponin 1 (basic Calponin), Calponin 2 (neutral Calponin), and Calponin 3 (acidic Calponin)—are predominantly expressed in smooth muscle tissue, with Calponin 2 also present in heart muscle and Calponin 3 found in the brain, highlighting the diverse roles across different tissues. Calponin 1/2/3 (H-3) antibody is an essential tool for researchers studying smooth muscle physiology and related pathologies.

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Calponin 1/2/3 Antibody (H-3) References:

1. Developmentally regulated expression of calponin isoforms and the effect of h2-calponin on cell proliferation.  |  Hossain, MM., et al. 2003. Am J Physiol Cell Physiol. 284: C156-67. PMID: 12388067
2. Mapping the microtubule binding regions of calponin.  |  Fattoum, A., et al. 2003. Biochemistry. 42: 1274-82. PMID: 12564930
3. Expression and purification of the h1 and h2 isoforms of calponin.  |  Jin, JP., et al. 2003. Protein Expr Purif. 31: 231-9. PMID: 14550641
4. Tyrosine phosphorylation of calponins. Inhibition of the interaction with F-actin.  |  Abouzaglou, J., et al. 2004. Eur J Biochem. 271: 2615-23. PMID: 15206927
5. A direct interaction with calponin inhibits the actin-nucleating activity of gelsolin.  |  Ferjani, I., et al. 2006. Biochem J. 396: 461-8. PMID: 16536729
6. Calponin. Developmental isoforms and a low molecular weight variant.  |  Draeger, A., et al. 1991. FEBS Lett. 291: 24-8. PMID: 1936245
7. A Conditional Knockout Mouse Model Reveals That Calponin-3 Is Dispensable for Early B Cell Development.  |  Flemming, A., et al. 2015. PLoS One. 10: e0128385. PMID: 26046660
8. Calponin isoforms CNN1, CNN2 and CNN3: Regulators for actin cytoskeleton functions in smooth muscle and non-muscle cells.  |  Liu, R. and Jin, JP. 2016. Gene. 585: 143-153. PMID: 26970176
9. Deletion of Calponin 2 in Mouse Fibroblasts Increases Myosin II-Dependent Cell Traction Force.  |  Hossain, MM., et al. 2016. Biochemistry. 55: 6046-6055. PMID: 27733037
10. Expression of the smooth muscle calponin gene in human osteosarcoma and its possible association with prognosis.  |  Yamamura, H., et al. 1998. Int J Cancer. 79: 245-50. PMID: 9645345

The preferred Calponin 1 antibody is Calponin 1 Antibody (CALP): sc-58707.