Calpain 7 Antibody (B-7): sc-137227
- Calpain 7 Antibody (B-7) is a mouse monoclonal IgG1 κ Calpain 7 antibody provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of Calpain 7 of human origin
- Calpain 7 Antibody (B-7) is recommended for detection of Calpain 7 of human origin by WB, IP, IF and ELISA
- Anti-Calpain 7 Antibody (B-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Calpain 7 Antibody (B-7) for WB applications. These reagents are now offered in bundles with Calpain 7 Antibody (B-7) (see ordering information below).
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Calpain 7 Antibody (B-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects Calpain 7 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Calpain 7 antibody (B-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Calpain 7, also known as CAPN6 or PalBH, is a member of the non-EF-hand subfamily of calpains and is characterized by a unique structure, which includes a long N-terminal domain and a PalB homologous domain flanking the protease domain. This atypical calpain, which consists of 813 amino acids, exhibits only 26-35% sequence identity with other calpain family members, primarily within the protease domain, highlighting its divergence and potential specialized functions. Calpain 7 is predominantly expressed in the brain and is localized in both the cytoplasm and nucleus, with activated forms found exclusively in the nucleus. Unique structure and localization are crucial for Calpain 7′s role in cellular processes, particularly in neurodegenerative diseases. Upregulation of Calpain 7 in striatal or cortical tissues of Huntington′s Disease knock-in mice suggests involvement in disease onset, making Calpain 7 a significant target for research into therapeutic interventions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Calpain 7 Antibody (B-7) References:
- Diverse mRNA expression patterns of the mouse calpain genes Capn5, Capn6 and Capn11 during development. | Dear, TN. and Boehm, T. 1999. Mech Dev. 89: 201-9. PMID: 10559499
- The calpain family and human disease. | Huang, Y. and Wang, KK. 2001. Trends Mol Med. 7: 355-62. PMID: 11516996
- Structure, activation, and biology of calpain. | Suzuki, K., et al. 2004. Diabetes. 53 Suppl 1: S12-8. PMID: 14749260
- Inhibition of calpain cleavage of huntingtin reduces toxicity: accumulation of calpain/caspase fragments in the nucleus. | Gafni, J., et al. 2004. J Biol Chem. 279: 20211-20. PMID: 14981075
- Molecular diversity of erythrocyte calpastatin. | Takano, E., et al. 1991. Biomed Biochim Acta. 50: 517-21. PMID: 1801717
- Molecular diversity of calpastatin in human erythroid cells. | Takano, E., et al. 1993. Arch Biochem Biophys. 303: 349-54. PMID: 8512320
- Autolysis, Ca2+ requirement, and heterodimer stability in m-calpain. | Elce, JS., et al. 1997. J Biol Chem. 272: 11268-75. PMID: 9111030
- Regulation of the calpain-calpastatin system by membranes (review). | Kawasaki, H. and Kawashima, S. 1996. Mol Membr Biol. 13: 217-24. PMID: 9116760
- Calpains: intact and active? | Johnson, GV. and Guttmann, RP. 1997. Bioessays. 19: 1011-8. PMID: 9394623
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Calpain 7 Antibody (B-7) | sc-137227 | 200 µg/ml | $316.00 | |||
Calpain 7 Antibody (B-7): m-IgGκ BP-HRP Bundle | sc-521459 | 200 µg Ab, 40 µg BP | $354.00 | |||
Calpain 7 Antibody (B-7): m-IgG1 BP-HRP Bundle | sc-533493 | 200 µg Ab; 20 µg BP | $354.00 | |||
Calpain 7 Antibody (B-7) AC | sc-137227 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Calpain 7 Antibody (B-7) HRP | sc-137227 HRP | 200 µg/ml | $316.00 | |||
Calpain 7 Antibody (B-7) FITC | sc-137227 FITC | 200 µg/ml | $330.00 | |||
Calpain 7 Antibody (B-7) PE | sc-137227 PE | 200 µg/ml | $343.00 | |||
Calpain 7 Antibody (B-7) Alexa Fluor® 488 | sc-137227 AF488 | 200 µg/ml | $357.00 | |||
Calpain 7 Antibody (B-7) Alexa Fluor® 546 | sc-137227 AF546 | 200 µg/ml | $357.00 | |||
Calpain 7 Antibody (B-7) Alexa Fluor® 594 | sc-137227 AF594 | 200 µg/ml | $357.00 | |||
Calpain 7 Antibody (B-7) Alexa Fluor® 647 | sc-137227 AF647 | 200 µg/ml | $357.00 | |||
Calpain 7 Antibody (B-7) Alexa Fluor® 680 | sc-137227 AF680 | 200 µg/ml | $357.00 | |||
Calpain 7 Antibody (B-7) Alexa Fluor® 790 | sc-137227 AF790 | 200 µg/ml | $357.00 |