Calpain 5 Antibody (A-5): sc-271271
- Calpain 5 Antibody (A-5) is a mouse monoclonal IgG1 κ Calpain 5 antibody, cited in 5 publications, provided at 200 µg/ml
- raised against amino acids 1-105 mapping at the N-terminus of Calpain 5 of human origin
- Calpain 5 Antibody (A-5) is recommended for detection of Calpain 5 of mouse, rat, human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Calpain 5 Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Calpain 5 Antibody (A-5) for WB and IHC(P) applications. These reagents are now offered in bundles with Calpain 5 Antibody (A-5) (see ordering information below).
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Calpain 5 Antibody (A-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Calpain 5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Calpain 5 antibody (A-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Calpain 5, also known as HTRA3, is a member of the non-classical calpain subfamily and lacks EF-hand calcium-binding structures typically found in classic calpains. Calpain 5 primarily resides in the cytoplasm and nucleus, playing a crucial role in intracellular protein processing and signal transduction. Calpain 5 participates in various cellular processes, including apoptosis and cell cycle regulation, which maintain cellular homeostasis and respond to stress signals. A T-domain in Calpain 5, homologous to the Caenorhabditis elegans protein Tra-3, suggests involvement in sex determination, highlighting significance in developmental biology. Interactions with other cellular proteins emphasize Calpain 5′s importance in various signaling pathways, making Calpain 5 monoclonal antibody (A-5) an essential tool for studying these complex biological processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Calpain 5 Antibody (A-5) References:
- Gene structure, chromosomal localization, and expression pattern of Capn12, a new member of the calpain large subunit gene family. | Dear, TN., et al. 2000. Genomics. 68: 152-60. PMID: 10964513
- Capn5 is expressed in a subset of T cells and is dispensable for development. | Franz, T., et al. 2004. Mol Cell Biol. 24: 1649-54. PMID: 14749380
- Inhibition of calpain cleavage of huntingtin reduces toxicity: accumulation of calpain/caspase fragments in the nucleus. | Gafni, J., et al. 2004. J Biol Chem. 279: 20211-20. PMID: 14981075
- Molecular cloning and characterization of rat and human calpain-5. | Waghray, A., et al. 2004. Biochem Biophys Res Commun. 324: 46-51. PMID: 15464980
- Gene expression and phenotypic characterization of mouse heart after chronic constant or intermittent hypoxia. | Fan, C., et al. 2005. Physiol Genomics. 22: 292-307. PMID: 15928208
- Specific haplotypes of the CALPAIN-5 gene are associated with polycystic ovary syndrome. | González, A., et al. 2006. Hum Reprod. 21: 943-51. PMID: 16396936
- The expression of genes related to adipocyte differentiation in pigs. | Wang, HC., et al. 2006. J Anim Sci. 84: 1059-66. PMID: 16612007
- Calpain-5 gene variants are associated with diastolic blood pressure and cholesterol levels. | Sáez, ME., et al. 2007. BMC Med Genet. 8: 1. PMID: 17227582
- Diverse mRNA expression patterns of the mouse calpain genes Capn5, Capn6 and Capn11 during development. | Dear, TN. and Boehm, T. 1999. Mech Dev. 89: 201-9. PMID: 10559499
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Calpain 5 Antibody (A-5) | sc-271271 | 200 µg/ml | $316.00 | |||
Calpain 5 Antibody (A-5): m-IgG Fc BP-HRP Bundle | sc-527228 | 200 µg Ab; 10 µg BP | $354.00 | |||
Calpain 5 Antibody (A-5): m-IgG1 BP-HRP Bundle | sc-532601 | 200 µg Ab; 20 µg BP | $354.00 | |||
Calpain 5 Antibody (A-5) AC | sc-271271 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Calpain 5 Antibody (A-5) HRP | sc-271271 HRP | 200 µg/ml | $316.00 | |||
Calpain 5 Antibody (A-5) FITC | sc-271271 FITC | 200 µg/ml | $330.00 | |||
Calpain 5 Antibody (A-5) PE | sc-271271 PE | 200 µg/ml | $343.00 | |||
Calpain 5 Antibody (A-5) Alexa Fluor® 488 | sc-271271 AF488 | 200 µg/ml | $357.00 | |||
Calpain 5 Antibody (A-5) Alexa Fluor® 546 | sc-271271 AF546 | 200 µg/ml | $357.00 | |||
Calpain 5 Antibody (A-5) Alexa Fluor® 594 | sc-271271 AF594 | 200 µg/ml | $357.00 | |||
Calpain 5 Antibody (A-5) Alexa Fluor® 647 | sc-271271 AF647 | 200 µg/ml | $357.00 | |||
Calpain 5 Antibody (A-5) Alexa Fluor® 680 | sc-271271 AF680 | 200 µg/ml | $357.00 | |||
Calpain 5 Antibody (A-5) Alexa Fluor® 790 | sc-271271 AF790 | 200 µg/ml | $357.00 |