Calpain 1 Antibody (P-6): sc-81171

Calpain 1 Antibody (P-6) is a mouse monoclonal IgG1 antibody that detects Calpain 1 in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Calpain 1, also known as µ-calpain, is a crucial intracellular calcium-dependent protease that plays a significant role in the cleavage of cytoskeletal and submembranous proteins, thereby influencing essential cellular processes such as cell fusion, mitosis, and meiosis. Calpain 1 exists as a heterodimer composed of a small regulatory subunit and a large catalytic subunit, which is vital for enzymatic activity. Calpastatin regulates Calpain 1 by inhibiting proteolytic activity and membrane binding, highlighting the importance of post-translational modifications in modulating function. This regulation is critical in various physiological contexts, including spermatogenesis and the acrosome reaction, where precise control of proteolytic activity is necessary for successful fertilization. Calpain 1 has been shown to co-localize with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain, suggesting involvement in neuroinflammatory processes. anti-Calpain 1 antibody (P-6) serves as an essential tool for researchers studying the intricate roles of this protease in cellular dynamics and disease mechanisms.

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Calpain 1 Antibody (P-6) References:

1. Neuroprotection in glaucoma using calpain-1 inhibitors: regional differences in calpain-1 activity in the trabecular meshwork, optic nerve and implications for therapeutics.  |  Govindarajan, B., et al. 2008. CNS Neurol Disord Drug Targets. 7: 295-304. PMID: 18673213
2. Calpain-1 induces endoplasmic reticulum stress in promoting cardiomyocyte apoptosis following hypoxia/reoxygenation.  |  Zheng, D., et al. 2015. Biochim Biophys Acta. 1852: 882-92. PMID: 25660447
3. Mitochondrial Calpain-1 Disrupts ATP Synthase and Induces Superoxide Generation in Type 1 Diabetic Hearts: A Novel Mechanism Contributing to Diabetic Cardiomyopathy.  |  Ni, R., et al. 2016. Diabetes. 65: 255-68. PMID: 26470784
4. Calpain-1 is associated with adverse relapse free survival in breast cancer: a confirmatory study.  |  Pu, X., et al. 2016. Histopathology. 68: 1021-9. PMID: 26496999
5. Differential Activation of Calpain-1 and Calpain-2 following Kainate-Induced Seizure Activity in Rats and Mice.  |  Seinfeld, J., et al. 2016. eNeuro. 3: PMID: 27622212
6. Calpain-1 deletion impairs mGluR-dependent LTD and fear memory extinction.  |  Zhu, G., et al. 2017. Sci Rep. 7: 42788. PMID: 28202907
7. The Calcium-Dependent Protease Calpain-1 Links TRPC6 Activity to Podocyte Injury.  |  Verheijden, KAT., et al. 2018. J Am Soc Nephrol. 29: 2099-2109. PMID: 29954830
8. Calpain-1 ablation partially rescues disease-associated hallmarks in models of Machado-Joseph disease.  |  Weber, JJ., et al. 2020. Hum Mol Genet. 29: 892-906. PMID: 31960910
9. Calpain-1 C2L domain peptide protects mouse hippocampus-derived neuronal HT22 cells against glutamate-induced oxytosis.  |  Sugawara, M., et al. 2021. Biochem Biophys Rep. 27: 101101. PMID: 34430716
10. Ginsenoside Rg1 alleviates vascular remodeling in hypoxia-induced pulmonary hypertension mice through the calpain-1/STAT3 signaling pathway.  |  Ran, C., et al. 2024. J Ginseng Res. 48: 405-416. PMID: 39036731

The preferred Calpain 1 antibody is Calpain 1 Antibody (D-11): sc-271313.