Calnexin Antibody (6D195): sc-70481

Calnexin Antibody (6D195) is a mouse monoclonal IgG1 kappa antibody raised against a human hepatoma cell line, reactive with Calnexin in mouse, rat, and human samples. Calnexin, also known as CANX, IP90, or p88, is a calcium-binding chaperone protein localized in the endoplasmic reticulum (ER) membrane. Structurally, Calnexin is a type I integral membrane protein consisting of a large luminal domain, a single transmembrane helix, and a short cytoplasmic tail; this structure is crucial for its role in protein folding and quality control within the ER. The luminal domain contains a lectin site that specifically binds to monoglucosylated N-linked oligosaccharides on newly synthesized glycoproteins, assisting in their proper folding and assembly. By retaining incompletely folded or misfolded proteins within the ER, Calnexin prevents their premature export to the Golgi apparatus, ensuring that only properly folded proteins proceed through the secretory pathway. Dysregulation of Calnexin function can lead to ER stress and is implicated in various diseases, including neurodegenerative disorders. Anti-Calnexin antibody (6D195) is suitable for applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF), making this antibody a valuable tool for researchers studying protein folding, ER stress responses, and related cellular mechanisms.

The preferred Calnexin antibody is Calnexin Antibody (AF18): sc-23954.