CALM Antibody (A-2): sc-271224

CALM Antibody (A-2) is a mouse monoclonal IgG3 kappa light chain antibody that detects CALM protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-CALM antibody (A-2) is available as the non-conjugated form. CALM protein, also known as Phosphatidylinositol Binding Clathrin Assembly Protein, plays a crucial role in clathrin-mediated endocytosis, which is essential for the internalization of various receptors and nutrients into cells. CALM protein is primarily located in the cytoplasm and is involved in the assembly of clathrin-coated pits and vesicles, facilitating transport of cargo from the trans-Golgi network and plasma membrane through interactions with adapter protein complexes such as AP-1 and AP-2. CALM′s cellular location enables regulation of vesicle formation and trafficking dynamics, which are vital for maintaining cellular homeostasis and signaling. Additionally, CALM shares significant homology with AP180, a synapse-specific clathrin assembly protein, suggesting a role in synaptic function and plasticity. CALM monoclonal antibody (A-2) versatility in various applications makes this product an invaluable tool for researchers studying clathrin-mediated processes and their implications in cellular physiology and pathology.

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CALM Antibody (A-2) References:

1. Clathrin assembly lymphoid myeloid leukemia (CALM) protein: localization in endocytic-coated pits, interactions with clathrin, and the impact of overexpression on clathrin-mediated traffic.  |  Tebar, F., et al. 1999. Mol Biol Cell. 10: 2687-702. PMID: 10436022
2. A novel chromosomal inversion at 11q23 in infant acute myeloid leukemia fuses MLL to CALM, a gene that encodes a clathrin assembly protein.  |  Wechsler, DS., et al. 2003. Genes Chromosomes Cancer. 36: 26-36. PMID: 12461747
3. Effect of clathrin assembly lymphoid myeloid leukemia protein depletion on clathrin coat formation.  |  Meyerholz, A., et al. 2005. Traffic. 6: 1225-34. PMID: 16262731
4. Conservation and diversity in families of coated vesicle adaptins.  |  Ponnambalam, S., et al. 1990. J Biol Chem. 265: 4814-20. PMID: 1969413
5. Cloning and expression of gamma-adaptin, a component of clathrin-coated vesicles associated with the Golgi apparatus.  |  Robinson, MS. 1990. J Cell Biol. 111: 2319-26. PMID: 2126014
6. Structural and functional division into two domains of the large (100- to 115-kDa) chains of the clathrin-associated protein complex AP-2.  |  Kirchhausen, T., et al. 1989. Proc Natl Acad Sci U S A. 86: 2612-6. PMID: 2495531
7. Cloning of cDNAs encoding two related 100-kD coated vesicle proteins (alpha-adaptins).  |  Robinson, MS. 1989. J Cell Biol. 108: 833-42. PMID: 2564002
8. CALM supports clathrin-coated vesicle completion upon membrane tension increase.  |  Willy, NM., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 34155137
9. Expression and localization of alpha-adaptin isoforms.  |  Ball, CL., et al. 1995. J Cell Sci. 108 (Pt 8): 2865-75. PMID: 7593326
10. Clathrin assembly protein AP180: primary structure, domain organization and identification of a clathrin binding site.  |  Morris, SA., et al. 1993. EMBO J. 12: 667-75. PMID: 8440257
11. The t(10;11)(p13;q14) in the U937 cell line results in the fusion of the AF10 gene and CALM, encoding a new member of the AP-3 clathrin assembly protein family.  |  Dreyling, MH., et al. 1996. Proc Natl Acad Sci U S A. 93: 4804-9. PMID: 8643484
12. Endocytosis and molecular sorting.  |  Mellman, I. 1996. Annu Rev Cell Dev Biol. 12: 575-625. PMID: 8970738

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CALM Antibody (A-2) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-CALM Antibody (A-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CALM Antibody (A-2) Specifications

Alternate Product Name: PICALM

Product Clone Name: A-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 619-651

Epitope Target: CALM

Epitope Species: human

Product Reactivity/Detection: CALM

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for CALM Antibody (A-2): P: Blocking Peptide