CalciFluor™ Fluo-8H, AM
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CalciFluortrade Fluo-8H, AM has been developed to improve cell loading and calcium response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelength of maximum excitation at ~490 nm and maximum emission at ~520 nm. Similarly to Fluo 4 AM, the anionic carboxy groups are protected with acetoxy methyl esters allowing membrane permeability, which are subsequently removed by intracellular esterases - allowing Ca2+ detection. However CalciFluortrade Fluo-8, AM is efficiently loaded into cells at room temperature (whereas Fluo-3 and Fluo-4 require much more harsh loading conditions), and CalciFluortrade Fluo-8 is 2 times brighter than Fluo-4 and 4 times brighter than Fluo-3. These features, as well as a lower price, make CalciFluor™ Fluo-8, AM a superior in vivo calcium indicator.
CalciFluor™ Fluo-8H, AM References
- Kinetic characterization of novel NR2B antagonists using fluorescence detection of calcium flux. | Bednar, B., et al. 2004. J Neurosci Methods. 137: 247-55. PMID: 15262068
- A knock-in mouse model of N-terminal R420W mutation of cardiac ryanodine receptor exhibits arrhythmogenesis with abnormal calcium dynamics in cardiomyocytes. | Okudaira, N., et al. 2014. Biochem Biophys Res Commun. 452: 665-8. PMID: 25193700
- Calcium influx into mouse spermatozoa activated by solubilized mouse zona pellucida, monitored with the calcium fluorescent indicator, fluo-3. Inhibition of the influx by three inhibitors of the zona pellucida induced acrosome reaction: tyrphostin A48, pertussis toxin, and 3-quinuclidinyl benzilate. | Bailey, JL. and Storey, BT. 1994. Mol Reprod Dev. 39: 297-308. PMID: 7888169
- Flow cytometric measurement of calcium influx in murine T cell hybrids using Fluo-3 and an organic-anion transport inhibitor. | Baus, E., et al. 1994. J Immunol Methods. 173: 41-7. PMID: 8034984
- Improved four-color flow cytometry method using fluo-3 and triple immunofluorescence for analysis of intracellular calcium ion ([Ca2+]i) fluxes among mouse lymph node B- and T-lymphocyte subsets. | Greimers, R., et al. 1996. Cytometry. 23: 205-17. PMID: 8974866
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CalciFluor™ Fluo-8H, AM, 1 mg | sc-361827 | 1 mg | $413.00 |