Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Calbindin D28K Double Nickase Plasmid (h): sc-400718-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Calbindin D28K Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Calbindin D28K Double Nickase Plasmid (h) and Calbindin D28K Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CALB1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Calbindin D28K Antibody (D-4): sc-365360
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Calbindin D28K Double Nickase Plasmid (h) | sc-400718-NIC | 20 µg | $410.00 | |||
Calbindin D28K Double Nickase Plasmid (h2) | sc-400718-NIC-2 | 20 µg | $410.00 |
CALB1 encodes calbindin D28K, a high-affinity cytosolic Ca2+-binding protein that buffers intracellular calcium and shapes the amplitude and kinetics of Ca2+ transients. By modulating calcium-dependent signaling, it influences neuronal excitability, synaptic integration, and activity-dependent transcriptional programs, and it contributes to cellular resilience against Ca2+-mediated stress. Calbindin D28K is widely used as a molecular marker for specific neuronal populations and participates in processes linked to calcium homeostasis, mitochondrial function, and oxidative stress responses. Altered CALB1 expression patterns have been reported in neurological and neuropsychiatric research contexts, supporting its relevance for mechanistic studies of circuit function and calcium dysregulation.
Calbindin D28K Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CALB1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CALB1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CALB1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CALB1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.