CAF-1 p150 Antibody (p150-48): sc-32742
- CAF-1 p150 Antibody (p150-48) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against human CAF-1 purified from human 293 cell nuclei
- Anti-CAF-1 p150 Antibody (p150-48) is recommended for detection of CAF-1 p150 of mouse, rat and human origin by WB, IP, IF and IHC(P)
- CAF-1 p150 Antibody (p150-48) is available conjugated to agarose for IP; and to HRP for WB, IHC(P) and ELISA
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CAF-1 p150 Antibody (p150-48) for WB and IHC(P) applications. These reagents are now offered in bundles with CAF-1 p150 Antibody (p150-48) (see ordering information below).
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CAF-1 p150 Antibody (p150-48) is a mouse monoclonal IgG1 kappa light chain antibody that detects CAF-1 p150 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-CAF-1 p150 antibody (p150-48) is available in both conjugated and non-conjugated forms, providing versatility for various experimental needs. The chromatin assembly factor-1 (CAF-1) complex, which includes the p150 subunit, plays a crucial role in the assembly of nucleosomes by depositing newly synthesized and acetylated histones H3 and H4 into nascent chromatin during DNA replication. This process is vital for maintaining genomic integrity and regulating gene expression, as proper chromatin structure is essential for DNA accessibility and function. CAF-1 p150 is particularly important for the maintenance of heterochromatin, which is characterized by its densely coiled structure and low gene density, making CAF-1 p150 relatively inaccessible to DNA-modifying enzymes. During late S phase, CAF-1 p150 interacts directly with heterochromatin-associated proteins, such as HP1α, HP1β, and HP1γ, facilitating the orderly assembly of chromatin. As cells transition into mitosis, the dissociation of CAF-1 p150 and HP1 proteins from heterochromatin coincides with the phosphorylation of histone H3, highlighting the dynamic nature of chromatin remodeling during the cell cycle. The reassociation of HP1 proteins with chromatin at the end of mitosis, following the dephosphorylation of histone H3, underscores the importance of CAF-1 p150 in the regulation of chromatin structure and function throughout cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CAF-1 p150 Antibody (p150-48) References:
- Genomic sequence and expression analyses of human chromatin assembly factor 1 p150 gene. | Dong, H., et al. 2001. Gene. 264: 187-96. PMID: 11250073
- The methyl-CpG binding protein MBD1 interacts with the p150 subunit of chromatin assembly factor 1. | Reese, BE., et al. 2003. Mol Cell Biol. 23: 3226-36. PMID: 12697822
- Gene-specific transcriptional activation mediated by the p150 subunit of the chromatin assembly factor 1. | Lee, SB., et al. 2009. J Biol Chem. 284: 14040-9. PMID: 19324875
- The p150 subunit of CAF-1 causes association of SUMO2/3 with the DNA replication foci. | Uwada, J., et al. 2010. Biochem Biophys Res Commun. 391: 407-13. PMID: 19919826
- The p150 subunit of the histone chaperone Caf-1 interacts with the transcriptional repressor Gfi1. | Heyd, F., et al. 2011. Biochim Biophys Acta. 1809: 255-61. PMID: 21570500
- A separable domain of the p150 subunit of human chromatin assembly factor-1 promotes protein and chromosome associations with nucleoli. | Smith, CL., et al. 2014. Mol Biol Cell. 25: 2866-81. PMID: 25057015
- The role of the chromatin assembly complex (CAF-1) and its p60 subunit (CHAF1b) in homeostasis and disease. | Volk, A. and Crispino, JD. 2015. Biochim Biophys Acta. 1849: 979-86. PMID: 26066981
- Chromatin Assembly Factor 1 (CAF-1) facilitates the establishment of facultative heterochromatin during pluripotency exit. | Cheng, L., et al. 2019. Nucleic Acids Res. 47: 11114-11131. PMID: 31586391
- CAF-1/p150 promotes cell proliferation, migration, invasion and predicts a poor prognosis in patients with cervical cancer. | Yang, S., et al. 2020. Oncol Lett. 20: 2338-2346. PMID: 32782551
- Unorthodox PCNA Binding by Chromatin Assembly Factor 1. | Gopinathan Nair, A., et al. 2022. Int J Mol Sci. 23: PMID: 36232396
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CAF-1 p150 Antibody (p150-48) | sc-32742 | 200 µg/ml | $316.00 | |||
CAF-1 p150 Antibody (p150-48): m-IgG Fc BP-HRP Bundle | sc-526684 | 200 µg Ab; 10 µg BP | $354.00 | |||
CAF-1 p150 Antibody (p150-48): m-IgG1 BP-HRP Bundle | sc-532057 | 200 µg Ab; 20 µg BP | $354.00 | |||
CAF-1 p150 Antibody (p150-48) AC | sc-32742 AC | 500 µg/ml, 25% agarose | $416.00 | |||
CAF-1 p150 Antibody (p150-48) HRP | sc-32742 HRP | 200 µg/ml | $316.00 | |||
CAF-1 p150 Antibody (p150-48) Alexa Fluor® 488 | sc-32742 AF488 | 200 µg/ml | $357.00 | |||
CAF-1 p150 Antibody (p150-48) Alexa Fluor® 546 | sc-32742 AF546 | 200 µg/ml | $357.00 | |||
CAF-1 p150 Antibody (p150-48) Alexa Fluor® 594 | sc-32742 AF594 | 200 µg/ml | $357.00 | |||
CAF-1 p150 Antibody (p150-48) Alexa Fluor® 647 | sc-32742 AF647 | 200 µg/ml | $357.00 | |||
CAF-1 p150 Antibody (p150-48) Alexa Fluor® 680 | sc-32742 AF680 | 200 µg/ml | $357.00 | |||
CAF-1 p150 Antibody (p150-48) Alexa Fluor® 790 | sc-32742 AF790 | 200 µg/ml | $357.00 |