cadherin-26 CRISPR/Cas9 KO Plasmid (h): sc-401989
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- cadherin-26 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the cadherin-26 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cadherin-26 CRISPR/Cas9 KO Plasmid (h) | sc-401989 | 20 µg | $397.00 |
Overview
CDH26 encodes cadherin-26, a calcium-dependent cell–cell adhesion molecule within the cadherin superfamily that helps regulate epithelial architecture and intercellular junctional organization. By influencing adhesion dynamics and tissue barrier properties, cadherin-26 can modulate processes such as epithelial differentiation, contact-dependent signaling, and cellular migration. Altered CDH26 expression has been reported in inflammatory mucosal contexts, supporting its relevance for studying epithelial–immune interactions and barrier-associated pathophysiology.
cadherin-26 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CDH26 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CDH26 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CDH26 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish cadherin-26 protein expression.
This CRISPR knockout system enables efficient generation of CDH26-deficient cell models for investigation of cadherin-26 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CDH26 exon(s) critical for cadherin-26 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CDH26 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by cadherin-26 CRISPR/Cas9 KO Plasmid (h) and cadherin-26 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CDH26 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by cadherin-26 HDR Plasmid (h) and cadherin-26 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CDH26 homology arms to support homology-directed repair at defined CDH26 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.