cadherin-24 CRISPR/Cas9 KO Plasmid (h): sc-401990
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- cadherin-24 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the cadherin-24 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cadherin-24 CRISPR/Cas9 KO Plasmid (h) | sc-401990 | 20 µg | $397.00 |
Overview
CDH24 encodes cadherin-24, a calcium-dependent cell–cell adhesion protein that contributes to epithelial tissue organization by supporting adherens junction integrity and contact-dependent signaling. Through cadherin-mediated interactions with catenins and the actin cytoskeleton, cadherin-24 influences processes such as cell polarity, barrier function, and coordinated cell migration. Dysregulated cadherin expression patterns can alter intercellular cohesion and signaling networks linked to epithelial remodeling and invasion-associated phenotypes. CDH24 is therefore of interest for studying adhesion-dependent regulation of tissue architecture and disease-relevant changes in cellular behavior.
cadherin-24 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CDH24 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CDH24 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CDH24 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish cadherin-24 protein expression.
This CRISPR knockout system enables efficient generation of CDH24-deficient cell models for investigation of cadherin-24 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CDH24 exon(s) critical for cadherin-24 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CDH24 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by cadherin-24 CRISPR/Cas9 KO Plasmid (h) and cadherin-24 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CDH24 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by cadherin-24 HDR Plasmid (h) and cadherin-24 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CDH24 homology arms to support homology-directed repair at defined CDH24 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.