CA XI CRISPR/Cas9 KO Plasmid (m): sc-419451
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CA XI CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CA XI genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CA XI CRISPR/Cas9 KO Plasmid (m) | sc-419451 | 20 µg | $397.00 |
Overview
Mouse Car11 encodes carbonic anhydrase XI (CA XI), a member of the carbonic anhydrase family that is thought to contribute to cellular pH regulation and bicarbonate/CO₂ handling within metabolic microenvironments, although its catalytic activity is reported to be atypical compared with classical carbonic anhydrases. CA XI expression has been associated with neural tissues, implicating Car11 in processes linked to neuronal physiology and ion/pH homeostasis. Through effects on intracellular and extracellular acid–base balance, Car11 may intersect with pathways influencing cell metabolism, differentiation, and microenvironmental adaptation. Dysregulated pH control and altered carbonic anhydrase family expression are relevant to studies of neurobiology and disease-associated metabolic remodeling in experimental models.
CA XI CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Car11 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Car11 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Car11 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CA XI protein expression.
This CRISPR knockout system enables efficient generation of Car11-deficient cell models for investigation of CA XI signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Car11 exon(s) critical for CA XI function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Car11 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CA XI CRISPR/Cas9 KO Plasmid (m) and CA XI CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Car11 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CA XI HDR Plasmid (m) and CA XI HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Car11 homology arms to support homology-directed repair at defined Car11 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.