C8β CRISPR/Cas9 KO Plasmid (h): sc-408409
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C8β CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the C8β genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C8β CRISPR/Cas9 KO Plasmid (h) | sc-408409 | 20 µg | $397.00 |
Overview
C8B encodes complement component C8 beta chain (C8β), an essential subunit of the C8 heterotrimer (C8α, C8β, C8γ) that participates in terminal complement activation. Within the classical, lectin, and alternative complement cascades, C8β helps stabilize assembly of the membrane attack complex (MAC; C5b-9) on target membranes, promoting pore formation and lytic effector function. This activity contributes to innate immune defense, inflammatory signaling, and crosstalk with opsonization and coagulation-related processes. Altered terminal complement function, including MAC dysregulation, is associated with susceptibility to infections and immune-mediated tissue injury, supporting C8B as a relevant target for immunology and inflammation research.
C8β CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the C8B gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the C8B together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the C8B open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish C8β protein expression.
This CRISPR knockout system enables efficient generation of C8B-deficient cell models for investigation of C8β signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting C8B exon(s) critical for C8β function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple C8B genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by C8β CRISPR/Cas9 KO Plasmid (h) and C8β CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the C8B locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by C8β HDR Plasmid (h) and C8β HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by C8B homology arms to support homology-directed repair at defined C8B target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.