C530008M17Rik CRISPR/Cas9 KO Plasmid (m): sc-435830
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C530008M17Rik CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the C530008M17Rik genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C530008M17Rik CRISPR/Cas9 KO Plasmid (m) | sc-435830 | 20 µg | $397.00 |
Overview
C530008M17Rik encodes a mouse protein that remains incompletely characterized, with limited functional annotation available from current genomic resources. As a predicted gene product, it is often investigated in the context of transcriptomic and proteomic datasets to define cell type–specific expression patterns and potential subcellular roles. Emerging evidence from co-expression and genetic perturbation screens can help place C530008M17Rik within broader cellular processes such as differentiation, stress responses, or tissue-specific homeostasis, depending on the experimental system. Due to sparse mechanistic data, targeted knockout approaches are commonly used to clarify pathway involvement and potential links to phenotypes relevant to developmental biology and disease modeling.
C530008M17Rik CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the C530008M17Rik gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the C530008M17Rik together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the C530008M17Rik open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish C530008M17Rik protein expression.
This CRISPR knockout system enables efficient generation of C530008M17Rik-deficient cell models for investigation of C530008M17Rik signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting C530008M17Rik exon(s) critical for C530008M17Rik function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple C530008M17Rik genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by C530008M17Rik CRISPR/Cas9 KO Plasmid (m) and C530008M17Rik CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the C530008M17Rik locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by C530008M17Rik HDR Plasmid (m) and C530008M17Rik HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by C530008M17Rik homology arms to support homology-directed repair at defined C530008M17Rik target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.