C4BP Antibody (GMA-036): sc-65970

C4BP Antibody (GMA-036) is a mouse monoclonal IgG1 antibody that detects C4BP protein of human origin by western blotting (WB). Anti-C4BP antibody (GMA-036) is available as a non-conjugated format, specifically designed to facilitate the study of the complement system. C4BP plays a crucial role in regulating the complement pathway, which is essential for immune response and inflammation. C4BP (GMA-036) antibody acts as a fluid-phase complement inhibitor, preventing excessive activation that can lead to tissue damage and cell apoptosis. C4BP is composed of multiple subunits, including α and β chains, which work together to enhance the inactivation of complement component C4, thereby maintaining homeostasis within the immune system. The regulation of C4 activation is vital, as uncontrolled complement activity can contribute to various autoimmune diseases and inflammatory conditions. C4BP monoclonal antibody (GMA-036) enables researchers to gain insights into the mechanisms of complement regulation and its implications in health and disease, making this antibody an invaluable tool for studies focused on immune response and therapeutic interventions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

C4BP Antibody (GMA-036) References:

1. A cluster of positively charged amino acids in the C4BP alpha-chain is crucial for C4b binding and factor I cofactor function.  |  Blom, AM., et al. 1999. J Biol Chem. 274: 19237-45. PMID: 10383431
2. [C4BP (C4b-binding protein)].  |  Fujita, T. 1999. Nihon Rinsho. 57 Suppl: 65-7. PMID: 10635776
3. Human C4b-binding protein has overlapping, but not identical, binding sites for C4b and streptococcal M proteins.  |  Blom, AM., et al. 2000. J Immunol. 164: 5328-36. PMID: 10799895
4. Positively charged amino acids at the interface between alpha-chain CCP1 and CCP2 of C4BP are required for regulation of the classical C3-convertase.  |  Blom, AM., et al. 2000. Mol Immunol. 37: 445-53. PMID: 11090879
5. The interaction between anticoagulant protein S and complement regulatory C4b-binding protein (C4BP).  |  van de Poel, RH., et al. 2000. Trends Cardiovasc Med. 10: 71-6. PMID: 11150733
6. [Proteins C,S and C4bp--their structures and functions].  |  Suzuki, K., et al. 1992. Rinsho Ketsueki. 33: 741-4. PMID: 1433912
7. Complement regulation in human atherosclerotic coronary lesions. Immunohistochemical evidence that C4b-binding protein negatively regulates the classical complement pathway, and that C5b-9 is formed via the alternative complement pathway.  |  Oksjoki, R., et al. 2007. Atherosclerosis. 192: 40-8. PMID: 16846604
8. C4b-binding protein and factor H compensate for the loss of membrane-bound complement inhibitors to protect apoptotic cells against excessive complement attack.  |  Trouw, LA., et al. 2007. J Biol Chem. 282: 28540-28548. PMID: 17699521
9. Laminin G1 residues of protein S mediate its TFPI cofactor function and are competitively regulated by C4BP.  |  Teraz-Orosz, A., et al. 2022. Blood Adv. 6: 704-715. PMID: 34731882
10. C4BP(β-)-mediated immunomodulation attenuates inflammation in DSS-induced murine colitis and in myeloid cells from IBD patients.  |  Serrano, I., et al. 2023. Pharmacol Res. 197: 106948. PMID: 37806602
11. Molecular cloning and characterization of the cDNA coding for C4b-binding protein, a regulatory protein of the classical pathway of the human complement system.  |  Chung, LP., et al. 1985. Biochem J. 230: 133-41. PMID: 3840370
12. Human C4-binding protein. I. Isolation and characterization.  |  Scharfstein, J., et al. 1978. J Exp Med. 148: 207-22. PMID: 670886

The preferred C4BPβ antibody is C4BPβ Antibody (E-1): sc-514553.