C4BPα Double Nickase Plasmid (h): sc-404684-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C4BPα Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- C4BPα Double Nickase Plasmid (h) and C4BPα Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting C4BPA. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: C4BPα Antibody (D-5): sc-398720
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C4BPα Double Nickase Plasmid (h) | sc-404684-NIC | 20 µg | $410.00 | |||
C4BPα Double Nickase Plasmid (h2) | sc-404684-NIC-2 | 20 µg | $410.00 |
C4BPA encodes the alpha chain of C4b-binding protein (C4BPα), a soluble regulator of the classical and lectin complement pathways that binds C4b and accelerates decay of C3 convertases to limit complement amplification. By serving as a cofactor for factor I–mediated cleavage of C4b, C4BPα helps maintain immune homeostasis and protects host surfaces from excessive complement-mediated inflammation. C4BPα also participates in clearance of immune complexes and apoptotic material and can influence opsonization-dependent responses at the interface of innate and adaptive immunity. Dysregulated C4BPA/C4BPα activity has been linked to complement imbalance observed in autoimmune and inflammatory disorders and to tumor-associated immune evasion mechanisms studied in multiple cancer contexts.
C4BPα Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the C4BPA locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within C4BPA. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt C4BPA function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of C4BPA-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.