C4a CRISPR/Cas9 KO Plasmid (h): sc-402428
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C4a CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the C4a genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C4a CRISPR/Cas9 KO Plasmid (h) | sc-402428 | 20 µg | $397.00 |
Overview
Complement component 4A (C4A) encodes the C4a fragment generated during proteolytic activation of complement C4, a central step in the classical and lectin complement pathways. C4 cleavage supports formation of the C3 convertase and promotes downstream opsonization, inflammatory signaling, and clearance of immune complexes. Variation in C4A copy number and expression has been linked to immune dysregulation and altered complement activity, with relevance to autoimmunity and neuroinflammation-associated phenotypes. As a readout of complement activation, C4a is commonly used to study innate immune crosstalk with adaptive responses and tissue injury mechanisms.
C4a CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the C4A gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the C4A together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the C4A open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish C4a protein expression.
This CRISPR knockout system enables efficient generation of C4A-deficient cell models for investigation of C4a signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting C4A exon(s) critical for C4a function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple C4A genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by C4a CRISPR/Cas9 KO Plasmid (h) and C4a CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the C4A locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by C4a HDR Plasmid (h) and C4a HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by C4A homology arms to support homology-directed repair at defined C4A target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.