C3a Antibody (K13/16): sc-47688
- C3a Antibody (K13/16) is a mouse monoclonal IgG1 κ C3a antibody, cited in 4 publications, provided at 200 µg/ml
- raised against complement protein factor C3a of human origin
- recommended for detection of C3a, C3a(desArg) and C3 of human origin by WB, IP, IF and IHC(P); non cross-reactive with C4a or C5a of human origin
- See C3 (B-9): sc-28294 for C3a antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for C3a Antibody (K13/16) for WB and IHC(P) applications. These reagents are now offered in bundles with C3a Antibody (K13/16) (see ordering information below).
C3a Antibody (K13/16) is a mouse monoclonal IgG1 kappa light chain antibody raised against complement protein factor C3a of human origin. C3a, also known as anaphylatoxin C3a, is a key fragment derived from the post-translational cleavage of complement component 3 (C3), which is central to the activation of the complement system in innate immunity. This cleavage is a crucial post-translational modification that transforms inactive C3 into active fragments C3a and C3b, enabling them to participate in immune responses. C3a functions as a potent anaphylatoxin, binding to the C3a receptor (C3aR) on various immune cells to mediate inflammation, chemotaxis, and cytokine release. The generation of C3a through this modification is essential for initiating and regulating inflammatory responses against pathogens. Additionally, C3a can be further processed into C3a(desArg), also known as acylation-stimulating protein (ASP), which plays a role in lipid metabolism and energy homeostasis. Anti-C3a antibody (K13/16) detects C3a, C3a(desArg), and intact C3 of human origin without cross-reacting with other complement components like C4a or C5a in applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). By targeting these critical fragments, C3a (K13/16) antibody is an invaluable tool for researchers studying complement activation, immune regulation, and associated pathophysiological conditions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
C3a Antibody (K13/16) References:
- Modulation of C3a activity: internalization of the human C3a receptor and its inhibition by C5a. | Settmacher, B., et al. 1999. J Immunol. 162: 7409-16. PMID: 10358194
- Ligand-induced phosphorylation of anaphylatoxin receptors C3aR and C5aR is mediated by 'G protein-coupled receptor kinases. | Langkabel, P., et al. 1999. Eur J Immunol. 29: 3035-46. PMID: 10508278
- A role for the C3a anaphylatoxin receptor in the effector phase of asthma. | Humbles, AA., et al. 2000. Nature. 406: 998-1001. PMID: 10984054
- Human complement component C3: cDNA coding sequence and derived primary structure. | de Bruijn, MH. and Fey, GH. 1985. Proc Natl Acad Sci U S A. 82: 708-12. PMID: 2579379
- Mitogen-activated protein kinase activation requires two signal inputs from the human anaphylatoxin C5a receptor. | Buhl, AM., et al. 1995. J Biol Chem. 270: 19828-32. PMID: 7649993
- TNF-alpha-mediated expression of the receptor for anaphylatoxin C5a on neurons in experimental Listeria meningoencephalitis. | Stahel, PF., et al. 1997. J Immunol. 159: 861-9. PMID: 9218605
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C3a Antibody (K13/16) | sc-47688 | 200 µg/ml | $322.00 | |||
C3a Antibody (K13/16): m-IgG Fc BP-HRP Bundle | sc-538933 | 200 µg Ab; 10 µg BP | $361.00 | |||
C3a Antibody (K13/16): m-IgG1 BP-HRP Bundle | sc-541162 | 200 µg Ab; 20 µg BP | $361.00 | |||
C3a Antibody (K13/16) L | sc-47688 L | 200 µg/0.1 ml | $322.00 |