C20orf160 CRISPR/Cas9 KO Plasmid (h): sc-414493
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C20orf160 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the C20orf160 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C20orf160 CRISPR/Cas9 KO Plasmid (h) | sc-414493 | 20 µg | $397.00 |
Overview
CCM2L (C20orf160) encodes a scaffold-like protein related to CCM2 that is implicated in endothelial signaling and vascular stability through interactions with CCM pathway components. It has been linked to regulation of MAPK signaling and cytoskeletal organization, processes that influence cell–cell junction integrity, mechanotransduction, and responses to inflammatory cues. Expression and functional studies suggest relevance to angiogenesis and cardiovascular development, with potential connections to cerebral cavernous malformation biology via pathway overlap rather than direct pathogenic annotation. As a result, CCM2L is frequently investigated in models of endothelial homeostasis, barrier function, and stress-responsive signaling networks.
C20orf160 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CCM2L gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CCM2L together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CCM2L open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish C20orf160 protein expression.
This CRISPR knockout system enables efficient generation of CCM2L-deficient cell models for investigation of C20orf160 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CCM2L exon(s) critical for C20orf160 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CCM2L genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by C20orf160 CRISPR/Cas9 KO Plasmid (h) and C20orf160 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CCM2L locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by C20orf160 HDR Plasmid (h) and C20orf160 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CCM2L homology arms to support homology-directed repair at defined CCM2L target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.