Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
C1s Double Nickase Plasmid (h): sc-404794-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C1s Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- C1s Double Nickase Plasmid (h) and C1s Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting C1S. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: C1s Antibody (D-6): sc-365273
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C1s Double Nickase Plasmid (h) | sc-404794-NIC | 20 µg | $410.00 | |||
C1s Double Nickase Plasmid (h2) | sc-404794-NIC-2 | 20 µg | $410.00 |
C1S encodes complement component C1s, a modular serine protease that functions within the C1 complex to initiate the classical complement pathway. Upon activation, C1s cleaves C4 and C2 to generate the C3 convertase, linking immune complex recognition to downstream opsonization and inflammatory signaling. C1s activity intersects with proteolytic cascades, extracellular protein processing, and innate immune regulation, shaping cytokine responses and clearance of apoptotic material. Dysregulated classical complement activation and genetic variants affecting C1S have been associated with immune-mediated inflammation, susceptibility to recurrent infection, and complement-driven tissue injury phenotypes, supporting its relevance in immunology and vascular biology research.
C1s Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the C1S locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within C1S. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt C1S function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of C1S-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.