C1s Antibody (D-6): sc-365273
- C1s Antibody (D-6) is a mouse monoclonal IgG2b κ C1s antibody, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 140-208 mapping within an internal region of C1s of human origin
- C1s Antibody (D-6) is recommended for detection of mature C1 esterase and C1s precursor of human origin by WB, IP, IF and ELISA
- Anti-C1s Antibody (D-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to biotin for WB, IHC(P) and ELISA
- m-IgGκ BP-HRP is the preferred secondary detection reagent for C1s Antibody (D-6) for WB applications. This reagent is now offered in a bundle with C1s Antibody (D-6) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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C1s Antibody (D-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects C1s protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-C1s antibody (D-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. C1s protein plays a crucial role in the complement system, specifically as part of the C1 complex, which is essential for the initiation of the classical pathway of complement activation. This pathway is vital for immune responses, as C1s helps to opsonize pathogens, promote inflammation, and facilitate the clearance of immune complexes. The C1 complex, composed of C1q, C1r, and C1s, operates as a calcium-dependent trimolecular complex, where C1s is activated into a disulfide-linked heterodimer consisting of a heavy chain and a light chain. Understanding C1s function is important because defects in the gene encoding for C1s can lead to selective C1s deficiency, a condition associated with an increased risk of autoimmune diseases. Using C1s (D-6) antibody in research can provide insights into the mechanisms of complement activation and implications in various immunological disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
C1s Antibody (D-6) References:
- Complement C1s activation in degenerating articular cartilage of rheumatoid arthritis patients: immunohistochemical studies with an active form specific antibody. | Nakagawa, K., et al. 1999. Ann Rheum Dis. 58: 175-81. PMID: 10364916
- Crystal structure of the catalytic domain of human complement c1s: a serine protease with a handle. | Gaboriaud, C., et al. 2000. EMBO J. 19: 1755-65. PMID: 10775260
- Molecular basis of a selective C1s deficiency associated with early onset multiple autoimmune diseases. | Dragon-Durey, MA., et al. 2001. J Immunol. 166: 7612-6. PMID: 11390518
- X-ray structure of the Ca2+-binding interaction domain of C1s. Insights into the assembly of the C1 complex of complement. | Gregory, LA., et al. 2003. J Biol Chem. 278: 32157-64. PMID: 12788922
- Complement determinations in human disease. | Glovsky, MM., et al. 2004. Ann Allergy Asthma Immunol. 93: 513-22; quiz 523-5, 605. PMID: 15609759
- Complexes between C1q and C3 or C4: novel and specific markers for classical complement pathway activation. | Wouters, D., et al. 2005. J Immunol Methods. 298: 35-45. PMID: 15847795
- Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry. | Liu, T., et al. 2005. J Proteome Res. 4: 2070-80. PMID: 16335952
- Probing the C4-binding site on C1s with monoclonal antibodies. Evidence for a C4/C4b-binding site on the gamma-domain. | Matsumoto, M., et al. 1989. J Immunol. 142: 2743-50. PMID: 2522968
- Complete cDNA sequence of human complement Cls and close physical linkage of the homologous genes Cls and Clr. | Tosi, M., et al. 1987. Biochemistry. 26: 8516-24. PMID: 2831944
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C1s Antibody (D-6) | sc-365273 | 200 µg/ml | $322.00 | |||
C1s Antibody (D-6): m-IgGκ BP-HRP Bundle | sc-552342 | 200 µg Ab; 40 µg BP | $361.00 | |||
C1s Antibody (D-6) AC | sc-365273 AC | 500 µg/ml, 25% agarose | $424.00 | |||
C1s Antibody (D-6) HRP | sc-365273 HRP | 200 µg/ml | $322.00 | |||
C1s Antibody (D-6) FITC | sc-365273 FITC | 200 µg/ml | $336.00 | |||
C1s Antibody (D-6) PE | sc-365273 PE | 200 µg/ml | $349.00 | |||
C1s Antibody (D-6) Alexa Fluor® 488 | sc-365273 AF488 | 200 µg/ml | $364.00 | |||
C1s Antibody (D-6) Alexa Fluor® 546 | sc-365273 AF546 | 200 µg/ml | $364.00 | |||
C1s Antibody (D-6) Alexa Fluor® 594 | sc-365273 AF594 | 200 µg/ml | $364.00 | |||
C1s Antibody (D-6) Alexa Fluor® 647 | sc-365273 AF647 | 200 µg/ml | $364.00 | |||
C1s Antibody (D-6) Alexa Fluor® 680 | sc-365273 AF680 | 200 µg/ml | $364.00 | |||
C1s Antibody (D-6) Alexa Fluor® 790 | sc-365273 AF790 | 200 µg/ml | $364.00 | |||
C1s Antibody (D-6) B | sc-365273 B | 200 µg/ml | $326.00 |