Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
C1q-C Double Nickase Plasmid (h): sc-403618-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C1q-C Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- C1q-C Double Nickase Plasmid (h) and C1q-C Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting C1QC. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: C1q-C Antibody (A-12): sc-365301
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C1q-C Double Nickase Plasmid (h) | sc-403618-NIC | 20 µg | $410.00 | |||
C1q-C Double Nickase Plasmid (h2) | sc-403618-NIC-2 | 20 µg | $410.00 |
C1QC encodes the C chain of complement component C1q, a pattern-recognition molecule that initiates the classical complement pathway by binding immune complexes and altered self structures. C1q participates in opsonization, phagocytic clearance, and immune modulation, linking innate immune recognition to downstream complement activation and inflammatory signaling. In the central nervous system and peripheral tissues, C1q contributes to microglial synapse pruning, debris clearance, and regulation of cytokine milieu, connecting complement biology to neuroimmune communication. Dysregulated C1q/C1QC expression and complement activation are associated with inflammatory and autoimmune processes, neurodegeneration, and tumor-associated macrophage phenotypes, supporting mechanistic studies of complement-driven tissue remodeling.
C1q-C Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the C1QC locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within C1QC. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt C1QC function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of C1QC-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.