Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
C1INH Double Nickase Plasmid (h): sc-403065-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C1INH Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- C1INH Double Nickase Plasmid (h) and C1INH Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SERPING1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: C1INH Antibody (B-11): sc-377062
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C1INH Double Nickase Plasmid (h) | sc-403065-NIC | 20 µg | $410.00 | |||
C1INH Double Nickase Plasmid (h2) | sc-403065-NIC-2 | 20 µg | $410.00 |
SERPING1 encodes C1 esterase inhibitor (C1INH), a secreted serpin that irreversibly inhibits multiple serine proteases in the complement system and contact activation pathways, including C1r/C1s, MASP-1/2, plasma kallikrein, and factor XIIa. By constraining classical and lectin complement activation and limiting bradykinin generation, C1INH helps maintain vascular homeostasis and prevents inappropriate inflammatory amplification. Disrupted SERPING1 function perturbs complement and kinin signaling, with strong relevance to disorders characterized by dysregulated plasma protease activity, increased vascular permeability, and inflammatory edema phenotypes.
C1INH Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SERPING1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SERPING1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SERPING1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SERPING1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.