C12orf34 CRISPR/Cas9 KO Plasmid (h): sc-408987
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C12orf34 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the C12orf34 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C12orf34 CRISPR/Cas9 KO Plasmid (h) | sc-408987 | 20 µg | $397.00 |
Overview
FAM222A encodes the human protein C12orf34, a poorly characterized factor with emerging links to cellular homeostasis and stress-responsive signaling. Available transcriptomic and proteomic datasets suggest context-dependent expression and potential involvement in intracellular trafficking and proteostasis networks that interface with inflammation-related pathways. Altered FAM222A/C12orf34 expression has been reported in disease-associated molecular profiles, including neurodegeneration and cancer-related datasets, supporting continued investigation into its regulatory roles. Loss-of-function models are useful to clarify how C12orf34 contributes to pathway crosstalk affecting cell survival, signaling balance, and tissue-specific phenotypes.
C12orf34 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FAM222A gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FAM222A together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FAM222A open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish C12orf34 protein expression.
This CRISPR knockout system enables efficient generation of FAM222A-deficient cell models for investigation of C12orf34 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FAM222A exon(s) critical for C12orf34 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FAM222A genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by C12orf34 CRISPR/Cas9 KO Plasmid (h) and C12orf34 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FAM222A locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by C12orf34 HDR Plasmid (h) and C12orf34 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FAM222A homology arms to support homology-directed repair at defined FAM222A target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.