Yes Antibody (C-10): sc-46674

c-Yes Antibody (C-10) is a mouse monoclonal IgG1 antibody that detects c-Yes protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). c-Yes (C-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. c-Yes protein plays a crucial role in cellular signaling pathways, particularly in regulating cell proliferation and differentiation, making c-Yes an important target for cancer research. As a member of the Src family of kinases, c-Yes (C-10) monoclonal antibody recognizes a protein involved in transducing signals that influence various cellular processes, including motility and adhesion. c-Yes protein is primarily located in the cytoplasm and can translocate to the cell membrane, where c-Yes interacts with other signaling molecules, thereby modulating activity and function. Human c-Yes gene, which maps to chromosome 18p11.31-p11.21, encodes a 543 amino acid protein that shares over 80% homology with c-Src, highlighting the evolutionary conservation and functional significance of these kinases. Understanding c-Yes dynamics and interactions with specific proteins can provide insights into c-Yes role in oncogenic processes and potential therapeutic targets in cancer treatment.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Yes Antibody (C-10) References:

1. The SH3 and SH2 domains are capable of directing specificity in protein interactions between the non-receptor tyrosine kinases cSrc and cYes.  |  Summy, JM., et al. 2000. Oncogene. 19: 155-60. PMID: 10644991
2. Kinases of the Src family: structure and functions.  |  Tatosyan, AG. and Mizenina, OA. 2000. Biochemistry (Mosc). 65: 49-58. PMID: 10702640
3. Src kinase-mediated signaling in leukocytes.  |  Korade-Mirnics, Z. and Corey, SJ. 2000. J Leukoc Biol. 68: 603-13. PMID: 11073097
4. Selected glimpses into the activation and function of Src kinase.  |  Bjorge, JD., et al. 2000. Oncogene. 19: 5620-35. PMID: 11114743
5. SRC family kinases mediate epithelial Na+ channel inhibition by endothelin.  |  Gilmore, ES., et al. 2001. J Biol Chem. 276: 42610-7. PMID: 11560932
6. c-Yes tyrosine kinase is a potent suppressor of ES cell differentiation and antagonizes the actions of its closest phylogenetic relative, c-Src.  |  Zhang, X., et al. 2014. ACS Chem Biol. 9: 139-46. PMID: 23895624
7. Identification of type I and type II inhibitors of c-Yes kinase using in silico and experimental techniques.  |  Ramakrishnan, C., et al. 2018. J Biomol Struct Dyn. 36: 1566-1576. PMID: 28589758
8. Location of the c-yes gene on the human chromosome and its expression in various tissues.  |  Semba, K., et al. 1985. Science. 227: 1038-40. PMID: 2983418
9. c-Yes tyrosine kinase activity in human colon carcinoma.  |  Park, J., et al. 1993. Oncogene. 8: 2627-35. PMID: 7690925
10. Elevated c-yes tyrosine kinase activity in premalignant lesions of the colon.  |  Peña, SV., et al. 1995. Gastroenterology. 108: 117-24. PMID: 7806032
11. Insights into Src kinase functions: structural comparisons.  |  Williams, JC., et al. 1998. Trends Biochem Sci. 23: 179-84. PMID: 9612082
12. Genetic organization of human proto-oncogenes.  |  Sakaguchi, AY. 1983. Prog Clin Biol Res. 119: 93-103. PMID: 6191340

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Yes Antibody (C-10) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-Yes Antibody (C-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Yes Antibody (C-10) Specifications

Alternate Product Name: Tyrosine Protein Kinase Yes

Product Clone Name: C-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: 1-95

Epitope Target: c-Yes

Epitope Species: human

Product Reactivity/Detection: c-Yes p61

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for Yes Antibody (C-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin