Src Antibody (5D10C4): sc-130074

c-Src Antibody (5D10C4) is a mouse monoclonal IgG2a kappa light chain antibody raised against amino acids 10-193 of human c-Src, a non-receptor tyrosine kinase crucial for cellular signal transduction. c-Src mouse monoclonal antibody (5D10C4) recognizes an epitope within the specified immunogen sequence and demonstrates broad species cross-reactivity, detecting Src family kinases in mouse, rat, and human samples through Western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA) applications. The regulation of c-Src, also known as proto-oncogene tyrosine-protein kinase Src, involves sophisticated post-translational modifications that act as molecular switches controlling c-Src′s activity. A prime example of this regulation occurs through phosphorylation at two key tyrosine residues: phosphorylation at tyrosine 527 (Y527) maintains c-Src in a closed, inactive conformation, while dephosphorylation of this site allows c-Src to adopt an open, active state. The activation process becomes further enhanced through autophosphorylation at tyrosine 416 (Y416) within the kinase domain, which increases c-Src′s catalytic activity. These precisely controlled phosphorylation events enable c-Src to respond appropriately to cellular signals and regulate various processes including proliferation, differentiation, motility, and adhesion. When these post-translational modifications become dysregulated, the resulting aberrant activation of c-Src can contribute to oncogenesis and cancer progression. Anti-c-Src monoclonal antibody (5D10C4) provides researchers with a valuable tool for investigating these regulatory mechanisms and their roles in signal transduction pathways, cellular growth regulation, and cancer development.

The preferred Src antibody is Src Antibody (B-12): sc-8056.