Src Antibody (4F1E8): sc-130069

c-Src Antibody (4F1E8) is a mouse monoclonal IgG2b antibody raised against amino acids 10-193 of human c-Src, a non-receptor tyrosine kinase essential for cellular signal transduction. c-Src mouse monoclonal antibody (4F1E8) recognizes an epitope within the specified immunogen sequence and demonstrates broad species cross-reactivity, detecting Src family kinases in mouse, rat, and human samples through Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry on paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA) applications. The structural organization of c-Src reveals sophisticated regulation: an N-terminal myristoylation site anchors c-Src to the cell membrane, while unique, SH3, SH2, and kinase domains work together to control c-Src′s function. The SH3 and SH2 domains serve as molecular bridges, facilitating crucial protein-protein interactions that assemble signaling complexes, while the kinase domain executes c-Src′s enzymatic activity. This intricate structural arrangement determines how c-Src interacts with binding partners and responds to regulatory signals within cells. c-Src′s activity undergoes precise control through phosphorylation of specific tyrosine residues, as maintaining appropriate c-Src signaling is crucial for normal cellular function. When c-Src regulation fails, the resulting aberrant activation can promote uncontrolled cell growth and contribute to cancer progression. Through these diverse cellular roles, c-Src functions as a critical mediator of proliferation, differentiation, motility, and adhesion. Anti-c-Src monoclonal antibody (4F1E8) provides researchers with a valuable tool for investigating signal transduction pathways, cellular growth regulation, and the molecular mechanisms underlying oncogenesis.

The preferred Src antibody is Src Antibody (B-12): sc-8056.