Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
c-Rel Double Nickase Plasmid (h): sc-400478-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- c-Rel Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- c-Rel Double Nickase Plasmid (h) and c-Rel Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting REL. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: c-Rel Antibody (B-6): sc-6955
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
c-Rel Double Nickase Plasmid (h) | sc-400478-NIC | 20 µg | $410.00 | |||
c-Rel Double Nickase Plasmid (h2) | sc-400478-NIC-2 | 20 µg | $410.00 |
REL encodes c-Rel, a transcription factor of the NF-κB family that regulates inducible gene expression programs controlling lymphocyte activation, cytokine production, cell survival, and differentiation. c-Rel participates in canonical NF-κB signaling downstream of receptors such as the TCR/BCR, CD40, and Toll-like receptors, coordinating inflammatory and immune transcriptional networks. Dysregulated REL activity and altered NF-κB pathway control have been implicated in immune dysfunction and oncogenic transcriptional states, particularly in hematologic malignancies. As a DNA-binding regulator, c-Rel is frequently studied for its roles in stimulus-dependent chromatin engagement and context-specific transcriptional output.
c-Rel Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the REL locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within REL. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt REL function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of REL-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.