C-Nap1 CRISPR/Cas9 KO Plasmid (m): sc-421134
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C-Nap1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the C-Nap1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C-Nap1 CRISPR/Cas9 KO Plasmid (m) | sc-421134 | 20 µg | $397.00 |
Overview
Cep250 encodes C-Nap1, a centrosomal coiled-coil protein concentrated at the proximal ends of centrioles where it contributes to centrosome cohesion and organization of the pericentriolar material. C-Nap1 functions within cell-cycle–regulated centrosome dynamics, coordinating with kinase signaling and scaffold proteins to support bipolar spindle assembly, accurate chromosome segregation, and maintenance of microtubule architecture. Disruption of centrosome cohesion and spindle integrity is closely linked to genomic instability, altered cell division, and developmental or neurodegenerative phenotypes in model systems. In mouse cell and tissue studies, Cep250 is therefore a useful node for interrogating centrosome-associated pathways that influence proliferation, differentiation, and tissue homeostasis.
C-Nap1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cep250 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cep250 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cep250 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish C-Nap1 protein expression.
This CRISPR knockout system enables efficient generation of Cep250-deficient cell models for investigation of C-Nap1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cep250 exon(s) critical for C-Nap1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cep250 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by C-Nap1 CRISPR/Cas9 KO Plasmid (m) and C-Nap1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cep250 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by C-Nap1 HDR Plasmid (m) and C-Nap1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cep250 homology arms to support homology-directed repair at defined Cep250 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.