c-Myb CRISPR/Cas9 KO Plasmid (m): sc-421766

Overview

Myb encodes the transcription factor c-Myb, a nuclear DNA-binding regulator that controls gene expression programs required for proliferation, survival, and lineage commitment in hematopoietic and other progenitor compartments. c-Myb modulates cell-cycle progression and differentiation by coordinating transcriptional networks with cofactors and chromatin regulators, integrating signals that influence self-renewal and maturation. Dysregulated MYB activity has been linked to altered hematopoiesis and oncogenic transcriptional states in multiple malignancies, making it a common target for mechanistic studies of transformation and differentiation blockade. In mouse models, Myb perturbation is widely used to dissect developmental timing, stem/progenitor maintenance, and transcriptional circuitry underpinning disease-relevant phenotypes.

c-Myb CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Myb gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Myb together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Myb open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish c-Myb protein expression.

This CRISPR knockout system enables efficient generation of Myb-deficient cell models for investigation of c-Myb signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting Myb exon(s) critical for c-Myb function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple Myb genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by c-Myb CRISPR/Cas9 KO Plasmid (m) and c-Myb CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Myb locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by c-Myb HDR Plasmid (m) and c-Myb HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Myb homology arms to support homology-directed repair at defined Myb target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.