c-Mpl Antibody (E-7): sc-377417

c-Mpl Antibody (E-7) is a mouse monoclonal IgM kappa antibody that detects c-Mpl protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-c-Mpl antibody (E-7) is available as the non-conjugated form. c-Mpl protein, also known as thrombopoietin receptor, is crucial for regulating megakaryopoiesis, the process by which megakaryocytes develop and produce platelets from hematopoietic stem cells. c-Mpl is primarily located in hematopoietic tissues, including bone marrow, spleen, and fetal liver, where c-Mpl mediates the effects of thrombopoietin (TPO), a key cytokine in platelet production. Upon binding to TPO, c-Mpl activates Janus kinase family members, Tyk 2 and JAK2, leading to phosphorylation of Stat5 and Stat3, which translocate to the nucleus to initiate transcription of genes essential for megakaryocyte development and platelet formation. Proper functioning of c-Mpl is vital, as mutations in c-Mpl have been linked to serious hematological disorders, such as congenital amegakaryocytic thrombocytopenia and thrombocytopenia with absent radii syndrome, highlighting c-Mpl′s importance in maintaining normal blood cell levels and overall hemostatic balance.

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c-Mpl Antibody (E-7) References:

1. Role of the distal half of the c-Mpl intracellular domain in control of platelet production by thrombopoietin in vivo.  |  Luoh, SM., et al. 2000. Mol Cell Biol. 20: 507-15. PMID: 10611229
2. Defective c-Mpl signaling in the syndrome of thrombocytopenia with absent radii.  |  Ballmaier, M., et al. 1998. Stem Cells. 16 Suppl 2: 177-84. PMID: 11012189
3. Compound heterozygosity for two different amino-acid substitution mutations in the thrombopoietin receptor (c-mpl gene) in congenital amegakaryocytic thrombocytopenia (CAMT).  |  Tonelli, R., et al. 2000. Hum Genet. 107: 225-33. PMID: 11071383
4. C-Mannosylation of thrombopoietin receptor (c-Mpl) regulates thrombopoietin-dependent JAK-STAT signaling.  |  Sasazawa, Y., et al. Biochem Biophys Res Commun. 468: 262-8. PMID: 26505790
5. C-Cbl regulates c-MPL receptor trafficking and its internalization.  |  Märklin, M., et al. 2020. J Cell Mol Med. 24: 12491-12503. PMID: 32954656
6. c-Mpl-del, a c-Mpl alternative splicing isoform, promotes AMKL progression and chemoresistance.  |  Li, F., et al. 2022. Cell Death Dis. 13: 869. PMID: 36229456
7. PF4 activates the c-Mpl-Jak2 pathway in platelets.  |  Buka, RJ., et al. 2024. Blood. 143: 64-69. PMID: 37883794
8. Regulation of platelet activation in vitro by the c-Mpl ligand, thrombopoietin.  |  Chen, J., et al. 1995. Blood. 86: 4054-62. PMID: 7492761
9. Thrombopoietin (TPO) induces tyrosine phosphorylation and activation of STAT5 and STAT3.  |  Bacon, CM., et al. 1995. FEBS Lett. 370: 63-8. PMID: 7544303
10. TPO and IL-3 induce overlapping but distinct protein tyrosine phosphorylation in a myeloid precursor cell line.  |  Dorsch, M., et al. 1995. Biochem Biophys Res Commun. 214: 424-31. PMID: 7545904
11. Thrombopoietin, c-Mpl ligand, induces tyrosine phosphorylation of Tyk2, JAK2, and STAT3, and enhances agonists-induced aggregation in platelets in vitro.  |  Ezumi, Y., et al. 1995. FEBS Lett. 374: 48-52. PMID: 7589510
12. Thrombocytopenia in c-mpl-deficient mice.  |  Gurney, AL., et al. 1994. Science. 265: 1445-7. PMID: 8073287

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-c-Mpl Antibody (E-7) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-c-Mpl Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

c-Mpl Antibody (E-7) Specifications

Alternate Product Name: c-Mpl

Product Clone Name: E-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 531-569

Epitope Target: c-Mpl

Epitope Species: human

Product Reactivity/Detection: c-Mpl

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for c-Mpl Antibody (E-7): P: Blocking Peptide