c-Maf Double Nickase Plasmid (h): sc-410543-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- c-Maf Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- c-Maf Double Nickase Plasmid (h) and c-Maf Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MAF. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: c-Maf Antibody (6B8): sc-293420
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
c-Maf Double Nickase Plasmid (h) | sc-410543-NIC | 20 µg | $410.00 | |||
c-Maf Double Nickase Plasmid (h2) | sc-410543-NIC-2 | 20 µg | $410.00 |
MAF encodes the transcription factor c-Maf, a basic leucine zipper protein that binds Maf recognition elements to regulate cell fate decisions and tissue-specific differentiation programs. c-Maf integrates signals from cytokine and developmental pathways, shaping transcriptional networks that influence proliferation, stress responses, and immune cell polarization, including roles in T helper cell differentiation and macrophage function. Dysregulated MAF/c-Maf activity has been associated with oncogenic transcriptional programs and altered immune regulation, and it is also implicated in eye and neural developmental processes. These properties make c-Maf a useful node for dissecting transcriptional control of lineage specification, metabolic adaptation, and context-dependent gene expression in human model systems.
c-Maf Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MAF locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MAF. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MAF function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MAF-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.