c-Kit Antibody (E-3): sc-365504

c-Kit Antibody (E-3) is a mouse monoclonal IgG1 kappa light chain antibody specifically engineered to recognize c-Kit protein, a crucial transmembrane receptor tyrosine kinase located on the cell surface. anti-c-Kit antibody (E-3) targets an epitope mapped between amino acids 958-976 within the C-terminus of human c-Kit, ensuring high specificity and reliable detection across mouse, rat, and human species using western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) techniques. c-Kit protein, also known as CD117, plays an essential role in various cellular processes, including cell proliferation, differentiation, survival, and migration, by mediating signaling pathways critical for hematopoiesis, mast cell development, and stem cell maintenance. c-Kit′s strategic location on the cell membrane allows effective interaction with stem cell factor (SCF), facilitating vital communication between cells and their microenvironment, which is fundamental for normal physiological functions and immune responses. Additionally, dysregulation or mutations in c-Kit gene are closely associated with the development and progression of several cancers, such as gastrointestinal stromal tumors and acute myeloid leukemia, highlighting significance as a therapeutic target. c-Kit (E-3) antibody is available in both non-conjugated form and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatile options for researchers to investigate c-Kit′s role in both healthy and diseased states, thereby advancing understanding of contributions to cellular biology and potential in cancer treatment.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

c-Kit Antibody (E-3) References:

1. Thymus reconstitution by c-kit-expressing hematopoietic stem cells purified from adult mouse bone marrow.  |  de Vries, P., et al. 1992. J Exp Med. 176: 1503-9. PMID: 1281208
2. Evidence that hematopoietic stem cells express mouse c-kit but do not depend on steel factor for their generation.  |  Ikuta, K. and Weissman, IL. 1992. Proc Natl Acad Sci U S A. 89: 1502-6. PMID: 1371359
3. The kit ligand: a cell surface molecule altered in steel mutant fibroblasts.  |  Flanagan, JG. and Leder, P. 1990. Cell. 63: 185-94. PMID: 1698555
4. Monoclonal antibody YB5.B8 identifies the human c-kit protein product.  |  Lerner, NB., et al. 1991. Blood. 77: 1876-83. PMID: 1708291
5. The rat c-kit ligand, stem cell factor, induces the development of connective tissue-type and mucosal mast cells in vivo. Analysis by anatomical distribution, histochemistry, and protease phenotype.  |  Tsai, M., et al. 1991. J Exp Med. 174: 125-31. PMID: 1711559
6. Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand.  |  Yarden, Y., et al. 1987. EMBO J. 6: 3341-51. PMID: 2448137
7. The proto-oncogene c-kit encoding a transmembrane tyrosine kinase receptor maps to the mouse W locus.  |  Chabot, B., et al. 1988. Nature. 335: 88-9. PMID: 2457811
8. The dominant-white spotting (W) locus of the mouse encodes the c-kit proto-oncogene.  |  Geissler, EN., et al. 1988. Cell. 55: 185-92. PMID: 2458842
9. c-kit protein, a transmembrane kinase: identification in tissues and characterization.  |  Majumder, S., et al. 1988. Mol Cell Biol. 8: 4896-903. PMID: 2463468
10. A new acute transforming feline retrovirus and relationship of its oncogene v-kit with the protein kinase gene family.  |  Besmer, P., et al. Nature. 320: 415-21. PMID: 3007997

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-c-Kit Antibody (E-3) has 32 citations in a variety of scientific publications.

Primary antibodies like Anti-c-Kit Antibody (E-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

c-Kit Antibody (E-3) Specifications

Alternate Product Name: c-Kit

Product Clone Name: E-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 958-976

Epitope Target: c-Kit

Epitope Species: human

Product Reactivity/Detection: c-Kit p145

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 32 citations in various scientific publications

Available Conjugates/Forms for c-Kit Antibody (E-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin