c-Kit Antibody (E-1): sc-17806

c-Kit Antibody (E-1) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect human c-Kit by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). c-Kit monoclonal antibody (E-1) is raised against amino acids 23-322 near the N-terminus of c-Kit and is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, ensuring versatility for diverse experimental applications. c-Kit, also known as CD117, is a transmembrane receptor tyrosine kinase that plays a crucial role in regulating essential cellular processes such as chemotaxis, proliferation, apoptosis, and adhesion by interacting with stem cell factor (SCF). Post-translational modifications of c-Kit, including glycosylation and phosphorylation, are vital for proper function and regulation. Glycosylation of c-Kit is essential for stability, proper folding, and cell surface localization, which facilitates effective ligand binding and receptor dimerization. Phosphorylation of specific tyrosine residues within the kinase domain is critical for activating signal transduction pathways that drive cellular responses such as hematopoiesis, mast cell development, melanogenesis, and gametogenesis. These modifications ensure that c-Kit can accurately transduce extracellular signals into appropriate cellular responses, maintaining cellular homeostasis and normal physiological functions. anti-c-Kit antibody (E-1) effectively binds to and detects c-Kit in native and modified forms, making this antibody an invaluable tool for researchers studying signaling mechanisms, disease pathology, and targeted therapeutic interventions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

c-Kit Antibody (E-1) References:

1. Signal transduction via the stem cell factor receptor/c-Kit.  |  Rönnstrand, L. 2004. Cell Mol Life Sci. 61: 2535-48. PMID: 15526160
2. c-Kit expression in human normal and malignant stem cells prognostic and therapeutic implications.  |  Hassan, HT. 2009. Leuk Res. 33: 5-10. PMID: 18639336
3. Oncogenic signaling from the hematopoietic growth factor receptors c-Kit and Flt3.  |  Masson, K. and Rönnstrand, L. 2009. Cell Signal. 21: 1717-26. PMID: 19540337
4. c-Kit expression and mutations in phyllodes tumors of the breast.  |  Bose, P., et al. 2010. Anticancer Res. 30: 4731-6. PMID: 21115932
5. c-kit(+) cells adopt vascular endothelial but not epithelial cell fates during lung maintenance and repair.  |  Liu, Q., et al. 2015. Nat Med. 21: 866-8. PMID: 26168292
6. C-kit receptor immunopositive interstitial cells (Cajal-type) in the porcine reproductive tract.  |  Domino, M., et al. 2017. Acta Vet Scand. 59: 32. PMID: 28526042
7. Loss of c-Kit function impairs arteriogenesis in a mouse model of hindlimb ischemia.  |  Hernandez, DR., et al. 2018. Surgery. 163: 877-882. PMID: 29287914
8. Reassessment of c-Kit+ Cells for Cardiomyocyte Contribution in Adult Heart.  |  He, L., et al. 2019. Circulation. 140: 164-166. PMID: 31283370
9. Stabilization of c-KIT G-Quadruplex DNA Structures by the RNA Polymerase I Inhibitors BMH-21 and BA-41.  |  Mazzini, S., et al. 2019. Int J Mol Sci. 20: PMID: 31590335
10. Necrotizing enterocolitis and C-KIT.  |  Yamataka, A., et al. 1998. J Pediatr Surg. 33: 1682-5. PMID: 9856895

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-c-Kit Antibody (E-1) has 19 citations in a variety of scientific publications.

Primary antibodies like Anti-c-Kit Antibody (E-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

c-Kit Antibody (E-1) Specifications

Alternate Product Name: c-Kit

Product Clone Name: E-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 23-322

Epitope Target: c-Kit

Epitope Species: human

Product Reactivity/Detection: c-Kit

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for c-Kit Antibody (E-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred c-Kit antibody is c-Kit Antibody (E-3): sc-365504.