c-IAP2 Double Nickase Plasmid (h): sc-400762-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- c-IAP2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- c-IAP2 Double Nickase Plasmid (h) and c-IAP2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BIRC3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: c-IAP2 Antibody (Ac-71): sc-517317
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
c-IAP2 Double Nickase Plasmid (h) | sc-400762-NIC | 20 µg | $410.00 | |||
c-IAP2 Double Nickase Plasmid (h2) | sc-400762-NIC-2 | 20 µg | $410.00 |
BIRC3 encodes the human cellular inhibitor of apoptosis protein 2 (c-IAP2), an E3 ubiquitin ligase that regulates TNF receptor superfamily signaling by ubiquitinating RIPK1 and related adaptors to tune NF-κB activation, inflammatory outputs, and cell death decisions. Through its BIR domains and RING finger, c-IAP2 integrates ubiquitin-dependent control of apoptosis and necroptosis, shaping caspase activity and survival signaling in response to cytokines and stress. Dysregulated BIRC3 function or expression is associated with altered immune signaling and apoptosis resistance, and recurrent pathway perturbations are reported in hematologic malignancies and other cancers. As a node in ubiquitin–proteasome and TNF/NF-κB networks, BIRC3 is frequently investigated in studies of inflammatory signaling, cell survival checkpoints, and ubiquitin-mediated pathway rewiring.
c-IAP2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BIRC3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BIRC3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BIRC3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BIRC3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.