c-Fos Antibody (4) Discontinued polyclonal antibody

c-Fos Antibody (4): sc-52

c-Fos Antibody (4) is rated 4.8 out of 5 by 36.
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    • c-Fos Antibody (4) is a rabbit polyclonal IgG; 100 µg/ml; or goat IgG; 100 µg/ml, sc-52-G
    • epitope mapping at the N-terminus of c-Fos of human origin
    • Discontinued polyclonal antibody
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c-Fos (4) has been discontinued and replaced by c-Fos (E-8): sc-166940.

Ordering Information

c-Fos (E-8): sc-166940 [ Recommended monoclonal replacement for c-Fos (4) ]
PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
c-Fos Antibody (E-8) sc-166940 200 µg/ml $289.00
c-Fos Antibody (E-8) AC sc-166940 AC 500 µg/ml, 25% agarose $379.00
c-Fos Antibody (E-8) Alexa Fluor® 488 sc-166940 AF488 200 µg/ml $326.00
c-Fos Antibody (E-8) Alexa Fluor® 647 sc-166940 AF647 200 µg/ml $326.00
c-Fos Antibody (E-8) FITC sc-166940 FITC 200 µg/ml $302.00
c-Fos Antibody (E-8) HRP sc-166940 HRP 200 µg/ml $289.00
c-Fos (E-8) Blocking Peptide sc-166940 P 100 µg/0.5 ml $63.00
c-Fos Antibody (E-8) PE sc-166940 PE 200 µg/ml $314.00

can this antibody(polyclonal) work in passerine birds? If not can you suggest a c-fos antibody polyclonal/monoclonal working in bird brain?

Asked by: Anupama
Thank you for your question. This discontinued c-fos antibody has not been tested in avian samples and so that is not a recommended species. Unfortunately we do not have any avian reactive c-Fos antibodies at this time. We are still gathering data on bird reactivity. Check back for updates!
Answered by: Tech Service
Date published: 2020-01-31

I used immunofluorescence staining in the hypothalamus of rat, and as a result c-fos was expressed in the cytoplasm. It's not expressed in the nucleus. Is it correct?

Asked by: Anonymous
Thank you for your question. According to the Human Protein Atlas, c-Fos (FOS) is localized to the nucleoplasm. Nuclear and cytoplasmic expression may be detected in most tissues. https://www.proteinatlas.org/ENSG00000170345-FOS/pathology
Answered by: Technical Support
Date published: 2019-05-25

Do you know of another vendor who can make something similar to sc-52-R? I've tried your monoclonal and it doesn't not react with our fish tissue. We absolutely must use a polyclonal sample.

Asked by: Kevin0525
Thank you for your question. No, there are no other suppliers who are offering this now discontinued polyclonal antibody. Our mouse monoclonal antibody, sc-166940: c-Fos (E-8), has been published in zebrafish samples. You can search for these specific papers using our Product Citation Search on our website and limiting the search by species. Please follow the link below. https://www.scbt.com/scbt/misc/citationSearch.jsp
Answered by: Technical Support
Date published: 2019-01-26

Your new c-Fos (E-8) antibody is specific for an epitope mapping between amino acids 128-152. Can you tell me which epitope your discontinued antibody (SC-52) was specific for? We still have some SC-52 in the lab and need to know what it recognizes.

Asked by: bwiltgen
The exact peptide sequence used to develop this now discontinued polyclonal antibody is considered proprietary. Please contact our Technical Service for further support.
Answered by: Technical Support
Date published: 2020-03-30

Is the epitope for this antibody conserved in frog--either xenopus laevis or tropicalis?

Asked by: Lora S
Thanks for your question. Unfortunately we do not have information regarding use in frog samples with this discontinued polyclonal antibody. It is very widely reactive, so chances are great it would work just fine, but it has not been validated for that use.
Answered by: Technical Support
Date published: 2018-12-14

¿que dilucion tengo que usar para hacer WB con este anticuerpo? Gracias

Asked by: jd_lorente
Gracias por su pregunta. c-Fos (4): sc-52 ha sido discontinuado. Nosotros recomendamos comenzando con una dilucion de 1:200 para el protocolo de Western blot usando este anticuerpo. Thank you for your question. c-Fos (4): sc-52 has been discontinued. We recommend starting with a 1:200 dilution for Western blot with this antibody.
Answered by: Technical Support
Date published: 2018-12-06

Please: I need information about a substitute product in relation to anti-c FOS antibody # nO. cat: sc-52 rabbit x rat. Is there other simlar product?

Asked by: Telma Andrade
Thank you for your question. We have several c-Fos antibodies that would be great substitutions for detection of c-Fos. Either sc-8047 or sc-413 would be a great suggestion! Please contact our Technical Service Department by phone, e-mail or live chat or your local distributor if you have any further questions or if you would like to try a sample of one of these two monoclonal antibodies.
Answered by: Tech Service
Date published: 2017-08-30

What antibody is the best replacement for SC-52-G? I need to double label with another Rabbit primary antibody.

Asked by: vhnmu
Thank you for your question. Because the goat polyclonal antibody, c-Fos (4): sc-52-G is now discontinued, we highly recommend using our mouse monoclonal IgG1 antibody, c-Fos (E-8): sc-166940 as a replacement. This mouse IgG1 primary antibody is suitable for use in double labeling experiment with a rabbit primary antibody. For a limited time, we are offering a free 10 µg sample of c-Fos (E-8): sc-166940. Please contact our Technical Service Department to request a sample. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-05-15
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Rated 5 out of 5 by from Excellent antibody This antibody gives very good staining of c-Fos on mouse brain sections prepared on the vibratome from brains perfused-fixed with 4% paraformadehyde.
Date published: 2019-09-23
Rated 5 out of 5 by from Great antibody I used the anti-c-Fos to label neurons across the mouse brain in free floating slices. This antibody worked very well with 1:500 dilution.
Date published: 2019-07-01
Rated 5 out of 5 by from Specific antibody This antibody recognizaed an unique band in whole cell extract from human PC3 cells.
Date published: 2019-04-03
Rated 5 out of 5 by from 效果很好 去年买的这个抗体,今年年初的时候用这个抗体做的Western Blot,效果很不错,条带也很清楚!!
Date published: 2017-09-13
Rated 5 out of 5 by from a good antibody for IHC I used brain sections, and 1:200 dilution, it worked well in IHC.
Date published: 2017-04-05
Rated 4 out of 5 by from Good performance in mouse vibratome sections Good performance in mouse vibratome sections stained with DAB (with or without nickel chloride) or fluorescent antibodies. However we must disclose we experienced some problems with recent purchases of this antibody, so we are currently using: sc-271243
Date published: 2016-05-30
Rated 5 out of 5 by from This has worked well in IP This has worked well in IP.
Date published: 2015-11-12
Rated 5 out of 5 by from sc sc-52-G: Works great in IHC in human tissues.
Date published: 2015-10-26
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