Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
c-Fgr Double Nickase Plasmid (h): sc-400702-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- c-Fgr Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- c-Fgr Double Nickase Plasmid (h) and c-Fgr Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FGR. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: c-Fgr Antibody (B-8): sc-166079
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
c-Fgr Double Nickase Plasmid (h) | sc-400702-NIC | 20 µg | $410.00 | |||
c-Fgr Double Nickase Plasmid (h2) | sc-400702-NIC-2 | 20 µg | $410.00 |
FGR encodes c-Fgr, a non-receptor Src family tyrosine kinase enriched in myeloid-lineage cells where it couples immunoreceptor and integrin cues to intracellular phosphorylation cascades. c-Fgr contributes to regulation of leukocyte adhesion, migration, degranulation, and phagocytic signaling, interfacing with pathways such as Fc receptor signaling, ITAM-dependent kinase networks, and cytoskeletal remodeling modules including PI3K and MAPK/ERK. Through these functions, FGR is relevant to inflammatory signaling and innate immune cell activation states, and altered Src-family kinase activity has been associated with dysregulated hematopoietic and immune processes. Human c-Fgr is therefore widely studied in mechanisms of immune receptor cross-talk, signal amplification, and kinase-dependent control of cell motility.
c-Fgr Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FGR locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FGR. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FGR function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FGR-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.