Anti-C/EBP alpha Antibody (D-5) recommended for detection of C/EBP α p42 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-C/EBP α Antibody (D-5): sc-365318

Anti-C/EBP α Antibody (D-5) is rated 4.5 out of 5 by 24.
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Datasheets

    • Anti-C/EBP α Antibody (D-5) is a mouse monoclonal IgM κ C/EBP α antibody, cited in 42 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 2-25 at the N-terminus of C/EBP α of human origin
    • recommended for detection of C/EBP α p42 of mouse, rat and human origin by WB, IP, IF and ELISA
    • available conjugated to HRP (sc-365318 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; and to fluoroscein (sc-365318 FITC), 200 µg/ml, for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488 for IF, IHC(P) and FCM
    • TransCruz reagent for ChIP application (sc-365318 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of C/EBP α (D-5): sc-365318.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for C/EBP α Antibody (D-5). This work is in progress.
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Can the TransCruz reagent sc-365318 X be used for applications other than ChIP or Gel Shift, such as Western Blot?

Asked by: AbPolly
Thank you for your question. Yes, the X form of the antibody can be used for all the recommended applications - just remember to dilute further since it is 10 times more concentrated than the standard form.
Answered by: Technical Support 5
Date published: 2017-01-19
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Rated 5 out of 5 by from Great We use this antibody to detect C/EBPa expression in 3T3-L1 cells by western blotting. The results are great. We recommend this antibody to other researchers.
Date published: 2020-08-21
Rated 4 out of 5 by from Worked good for Western blot Used at dilution of 1:500 in 5%BSA/TBST overnight. The band was clear but with non-specific signal.
Date published: 2018-08-09
Rated 3 out of 5 by from Pretty good, lots of non-specific bands Used on a western blot using 100 ug of protein from tissue. ~42 kDa band was visible and strong, just many non-specific bands.
Date published: 2018-02-21
Rated 4 out of 5 by from the antibody is good I bought this antibody for CEBPa detection in 3T3L1 cells, bands are clear
Date published: 2018-01-30
Rated 5 out of 5 by from great antibody Worked very well for WB and IC on mouse and rat cardiomyocytes
Date published: 2017-12-14
Rated 5 out of 5 by from great We use this antibody to detect C/EBP expression in the U251 cells by western blot. The results are surprising. We recommend this antibody to the researcher.
Date published: 2017-11-03
Rated 5 out of 5 by from Strong signal at expected size We used this antibody at 1:200 dilution in 1% milk/TBS-T overnight. Signals shown are after 3 min of exposure to HRP/ECL reaction.
Date published: 2017-10-17
Rated 5 out of 5 by from great features use in NB4 cells ,the trips is very abvious,thank you for your free sample
Date published: 2017-09-06
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