BUP-1 Antibody (F-11): sc-374066
- BUP-1 Antibody (F-11) is a mouse monoclonal IgG2a κ BUP-1 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 277-311 within an internal region of BUP-1 of human origin
- BUP-1 Antibody (F-11) is recommended for detection of BUP-1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-BUP-1 Antibody (F-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for BUP-1 Antibody (F-11) for WB applications. These reagents are now offered in bundles with BUP-1 Antibody (F-11) (see ordering information below).
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BUP-1 Antibody (F-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects BUP-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). BUP-1 (F-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BUP-1, also known as β-ureidopropionase or N-carbamyl-β-alanine amidohydrolase, is a crucial enzyme in pyrimidine catabolism, specifically catalyzing the hydrolysis of N-carbamyl-β-aminoisobutyric acid to produce β-aminoisobutyric acid, ammonia, and carbon dioxide. This reaction represents the final step in thymine and uracil degradation, highlighting BUP-1′s essential role in nucleotide metabolism. Proper functioning of BUP-1 is vital, as deficiencies can lead to toxic metabolite accumulation, resulting in elevated N-carbamyl-β-aminoisobutyric acid and N-carbamyl-β-alanine levels in biological fluids, which may contribute to neurological disorders. BUP-1 is predominantly found in liver and kidney cell cytoplasm, where BUP-1 plays a significant role in maintaining metabolic homeostasis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
BUP-1 Antibody (F-11) References:
- cDNA cloning, genomic structure and chromosomal localization of the human BUP-1 gene encoding beta-ureidopropionase. | Vreken, P., et al. 1999. Biochim Biophys Acta. 1447: 251-7. PMID: 10542323
- Expression and properties of human liver beta-ureidopropionase. | Sakamoto, T., et al. 2001. J Nutr Sci Vitaminol (Tokyo). 47: 132-8. PMID: 11508704
- beta-Ureidopropionase deficiency: a novel inborn error of metabolism discovered using NMR spectroscopy on urine. | Moolenaar, SH., et al. 2001. Magn Reson Med. 46: 1014-7. PMID: 11675655
- Confirmation of the enzyme defect in the first case of beta-ureidopropionase deficiency. Beta-alanine deficiency. | van Kuilenburg, AB., et al. 2000. Adv Exp Med Biol. 486: 243-6. PMID: 11783492
- beta-Ureidopropionase deficiency: an inborn error of pyrimidine degradation associated with neurological abnormalities. | van Kuilenburg, AB., et al. 2004. Hum Mol Genet. 13: 2793-801. PMID: 15385443
- Beta-ureidopropionase deficiency presenting with febrile status epilepticus. | Assmann, BE., et al. 2006. Epilepsia. 47: 215-7. PMID: 16417553
- Clinical findings and a therapeutic trial in the first patient with beta-ureidopropionase deficiency. | Assmann, B., et al. 2006. Neuropediatrics. 37: 20-5. PMID: 16541364
- Genetic analysis of the first 4 patients with beta-ureidopropionase deficiency. | van Kuilenburg, AB., et al. 2006. Nucleosides Nucleotides Nucleic Acids. 25: 1093-8. PMID: 17065070
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BUP-1 Antibody (F-11) | sc-374066 | 200 µg/ml | $322.00 | |||
BUP-1 Antibody (F-11): m-IgG Fc BP-HRP Bundle | sc-525936 | 200 µg Ab; 10 µg BP | $361.00 | |||
BUP-1 Antibody (F-11): m-IgG2a BP-HRP Bundle | sc-546464 | 200 µg Ab; 10 µg BP | $361.00 | |||
BUP-1 Antibody (F-11) AC | sc-374066 AC | 500 µg/ml, 25% agarose | $424.00 | |||
BUP-1 Antibody (F-11) HRP | sc-374066 HRP | 200 µg/ml | $322.00 | |||
BUP-1 Antibody (F-11) FITC | sc-374066 FITC | 200 µg/ml | $336.00 | |||
BUP-1 Antibody (F-11) PE | sc-374066 PE | 200 µg/ml | $349.00 | |||
BUP-1 Antibody (F-11) Alexa Fluor® 488 | sc-374066 AF488 | 200 µg/ml | $364.00 | |||
BUP-1 Antibody (F-11) Alexa Fluor® 546 | sc-374066 AF546 | 200 µg/ml | $364.00 | |||
BUP-1 Antibody (F-11) Alexa Fluor® 594 | sc-374066 AF594 | 200 µg/ml | $364.00 | |||
BUP-1 Antibody (F-11) Alexa Fluor® 647 | sc-374066 AF647 | 200 µg/ml | $364.00 | |||
BUP-1 Antibody (F-11) Alexa Fluor® 680 | sc-374066 AF680 | 200 µg/ml | $364.00 | |||
BUP-1 Antibody (F-11) Alexa Fluor® 790 | sc-374066 AF790 | 200 µg/ml | $364.00 | |||
BUP-1 (F-11) Neutralizing Peptide | sc-374066 P | 100 µg/0.5 ml | $69.00 |