BUB3 Antibody (E-7): sc-376506

BUB3 Antibody (E-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects BUB3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). BUB3 (E-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BUB3 protein, also known as budding uninhibited by benzimidazoles 3 homolog, BUB3L, or hBUB3, is a critical component of the mitotic spindle assembly complex, which plays a vital role in ensuring accurate chromosome segregation during cell division. BUB3 contains five WD repeat domains and is essential for the proper localization of BUB1 and BUBR1 at the kinetochore, thereby facilitating the spindle checkpoint pathway that prevents the onset of anaphase until all chromosomes are correctly attached to the spindle apparatus. This checkpoint is crucial during early embryogenesis, as BUB3 safeguards against chromosomal abnormalities that could lead to developmental issues or diseases such as cancer. Additionally, BUB3 interacts with Tctex1L (or DYNLT3), a dynein light chain, highlighting BUB3′s involvement in the regulation of microtubule dynamics and the overall fidelity of mitosis.

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BUB3 Antibody (E-7) References:

1. Retention of the BUB3 checkpoint protein on lagging chromosomes.  |  Martinez-Exposito, MJ., et al. 1999. Proc Natl Acad Sci U S A. 96: 8493-8. PMID: 10411903
2. hBUB1 defects in leukemia and lymphoma cells.  |  Ru, HY., et al. 2002. Oncogene. 21: 4673-9. PMID: 12096343
3. Distinct chromosome segregation roles for spindle checkpoint proteins.  |  Warren, CD., et al. 2002. Mol Biol Cell. 13: 3029-41. PMID: 12221113
4. The DYNLT3 light chain directly links cytoplasmic dynein to a spindle checkpoint protein, Bub3.  |  Lo, KW., et al. 2007. J Biol Chem. 282: 11205-12. PMID: 17289665
5. Inhibitory factors associated with anaphase-promoting complex/cylosome in mitotic checkpoint.  |  Braunstein, I., et al. 2007. Proc Natl Acad Sci U S A. 104: 4870-5. PMID: 17360335
6. The human spindle assembly checkpoint protein Bub3 is required for the establishment of efficient kinetochore-microtubule attachments.  |  Logarinho, E., et al. 2008. Mol Biol Cell. 19: 1798-813. PMID: 18199686
7. A motif from Lys216 to Lys222 in human BUB3 protein is a nuclear localization signal and critical for BUB3 function in mitotic checkpoint.  |  Zhu, S., et al. 2015. J Biol Chem. 290: 11282-92. PMID: 25814666
8. SETD1A function in leukemia is mediated through interaction with mitotic regulators BuGZ/BUB3.  |  Perlee, S., et al. 2023. EMBO Rep. 24: e57108. PMID: 37535603
9. YY2/BUB3 Axis promotes SAC Hyperactivation and Inhibits Colorectal Cancer Progression via Regulating Chromosomal Instability.  |  Hosea, R., et al. 2024. Adv Sci (Weinh). 11: e2308690. PMID: 38682484
10. Suppression of a conditional mutation in alpha-tubulin by overexpression of two checkpoint genes.  |  Guénette, S., et al. 1995. J Cell Sci. 108 (Pt 3): 1195-204. PMID: 7622604
11. The Saccharomyces cerevisiae checkpoint gene BUB1 encodes a novel protein kinase.  |  Roberts, BT., et al. 1994. Mol Cell Biol. 14: 8282-91. PMID: 7969164
12. Bub1p kinase activates the Saccharomyces cerevisiae spindle assembly checkpoint.  |  Farr, KA. and Hoyt, MA. 1998. Mol Cell Biol. 18: 2738-47. PMID: 9566893

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-BUB3 Antibody (E-7) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-BUB3 Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

BUB3 Antibody (E-7) Specifications

Alternate Product Name: BUB3

Product Clone Name: E-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 229-328

Epitope Target: BUB3

Epitope Species: human

Product Reactivity/Detection: BUB3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for BUB3 Antibody (E-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin