BTEB1 Double Nickase Plasmid (m): sc-421299-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BTEB1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BTEB1 Double Nickase Plasmid (m) and BTEB1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Klf9. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BTEB1 Antibody (A-5): sc-376422
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BTEB1 Double Nickase Plasmid (m) | sc-421299-NIC | 20 µg | $410.00 | |||
BTEB1 Double Nickase Plasmid (m2) | sc-421299-NIC-2 | 20 µg | $410.00 |
Klf9 encodes BTEB1 (Kruppel-like factor 9), a C2H2 zinc-finger transcription factor that binds GC-rich promoter elements to regulate context-dependent gene expression programs. In mouse cells, BTEB1 integrates signals from nuclear hormone and stress-responsive pathways to modulate differentiation, cell-cycle control, and metabolic homeostasis, often through interactions with Sp/KLF transcriptional networks and chromatin regulators. Klf9 activity has been linked to neuronal maturation, endocrine-responsive transcription, and tissue remodeling processes, making it relevant for studies of developmental biology and stimulus-dependent transcriptional rewiring. Dysregulated Klf9/BTEB1 expression has been associated with altered proliferation and differentiation states in disease-relevant models, supporting its use as a node for mechanistic pathway interrogation.
BTEB1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Klf9 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Klf9. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Klf9 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Klf9-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.