Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BSPII Double Nickase Plasmid (h): sc-402368-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BSPII Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BSPII Double Nickase Plasmid (h) and BSPII Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IBSP. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BSPII Antibody (LFMb-24): sc-73634
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BSPII Double Nickase Plasmid (h) | sc-402368-NIC | 20 µg | $410.00 | |||
BSPII Double Nickase Plasmid (h2) | sc-402368-NIC-2 | 20 µg | $410.00 |
Integrin-binding sialoprotein (IBSP), also known as bone sialoprotein II (BSPII), is a secreted, highly phosphorylated SIBLING family glycoprotein enriched in mineralized tissues and deposited in the extracellular matrix. Through its RGD motif and interactions with hydroxyapatite, BSPII promotes osteoblast adhesion, matrix organization, and nucleation of hydroxyapatite during bone formation and remodeling. IBSP activity interfaces with integrin-mediated signaling and extracellular matrix remodeling programs that influence cell migration and osteogenic differentiation. Dysregulated IBSP/BSPII expression has been associated with altered bone turnover and is frequently examined as a marker and functional regulator in studies of tumor–bone interactions and bone metastasis biology.
BSPII Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IBSP locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IBSP. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IBSP function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IBSP-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.